100 高等植物において情報伝達に関与するGTP結合タンパク質(ポスター発表の部)

概要

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In connection with studies of the molecular mechanism of signal-transduction pathway in plant cells, occurrence of a GTP-binding protein (G protein) in the seedling of soybean (Glycine max) was investigated. Proteins from the seedling of soybean were subjected to SDS-PAGE and Western blot analysis with antibody raised against a GTP binding site of G protein in animal. The antibody was found to bound specifically to a 40 kDa protein (p40). [γ-^<35>S]GPT was incorpolated into p40 specifically. These results show that the G protein is present in seeding of soybean. On the other hand, incorporation of [5-^3H]mevalonic acid to the seedling resulted in formation of radioactive p40, which was hydrolyzed to give radioactive geranylgeraniol. This observation shows that p40 is modified by the C_<20>-prenyl chain.
天然有機化合物討論会の論文
1992-09-10