88(P79) CH-,CH_2-,CH_3-選択二次元NMRスペクトルとその応用(ポスター発表の部)

概要

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The phase sensitive CH- and CH_2-selected 2D spectra of HSQC, HSQC-TOCSY, HSQC-relay, and HSQC-ROESY spectra were obtained by setting proper delays and ^1H-pulse angle of the editing sequence in E-HSQC followed by a suitable magnetization transfer if necessary. The magnitude mode CH_3-selected HMQC-TOCSY spectrum was obtained by applying a quadruple quantum fitter using a pulsed field gradient. These methods solved the cancellation problem caused by overlapping of the opposite phase peaks and also reached the relatively high resolution in F_1 axis by limiting the F_1 spectral width. Since in a certain total measurement time, the narrower is the F_1 spectral width which can reduce the data points in F_1 axis (t_1 increments), the larger number of transients can be accumulated, the C/H correlation methods including the insensitive magnetization transfer such as ROESY become applicable to natural products of small amount. Application of these methods for analysis of complex natural products such as oligosaccharides and a peptide in which signal overlapping is severe are also presented.
天然有機化合物討論会の論文
1998-08-31