High-Level Expression of Recombinant Human Nerve Growth Factor Beta in Milk of Nontransgenic Rabbits(MISCELLANEOUS)
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概要
- 論文の詳細を見る
The technology for the large-scale production of therapeutic recombinant proteins remains a challenge in the biopharmaceutical industry. In this study, we reported a nontransgenic approach to producing a large quantity of human nerve growth factor beta(hNGF-β) in rabbit milk by employing a recombinant adenoviral expression system. After directly instilling hNGF-β recombinant adenoviruses into rabbit mammary glands, a polypeptide with a molecular weight of 13.2 kDa was detected in rabbit milk. The maximal expression level of hNGF-β reached 346 μg/ml. The biological activity of recombinant hNGF-β was confirmed using PC12 cells and cultures of dorsal root ganglion neurons from chicken embryos. Our data suggest that instilling recombinant adenovirus directly into the mammary gland of mammals is an efficient approach to producing a large quantity of hNGF-β.
- 社団法人日本生物工学会の論文
- 2008-04-25
著者
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Li Jian
Department Of Structural Engineering
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Li Kun
Department Of Biological Engineering College Of Environment & Chemical Engineering Yanshan Unive
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Li Kun
Department Of Applied Physics And Materials Research Center The Hong Kong Polytechnic University
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Xiao Bo
College Of Animal Science And Technology Northwest A&f University:college Of Life Sciences Ludon
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Li QingWang
College of Animal Science and Technology, Northwest A&F University
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Feng Bo
Department of Biological Engineering, College of Environment & Chemical Engineering, Yanshan Univers
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Han ZengSheng
Department of Biological Engineering, College of Environment & Chemical Engineering, Yanshan Univers
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Gao DaWei
Department of Biological Engineering, College of Environment & Chemical Engineering, Yanshan Univers
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Zhao Rui
Department of Biological Engineering, College of Environment & Chemical Engineering, Yanshan Univers
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Jiang ZhongLiang
College of Animal Science and Technology, Northwest A&F University
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Hu JianHong
College of Animal Science and Technology, Northwest A&F University
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Zhi XuBo
College of Animal Science and Technology, Northwest A&F University
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Feng Bo
Department Of Biological Engineering College Of Environment & Chemical Engineering Yanshan Unive
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Li Jian
Department Of Biological Engineering College Of Environment & Chemical Engineering Yanshan Unive
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Zhi Xubo
College Of Animal Science And Technology Northwest A&f University
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Zhao Rui
Department Of Biological Engineering College Of Environment & Chemical Engineering Yanshan Unive
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Gao Dawei
Department Of Biological Engineering College Of Environment & Chemical Engineering Yanshan Unive
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Hu Jianhong
College Of Animal Science And Technology Northwest A&f University
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Li Qingwang
中華人民共和国
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Li Qingwang
College Of Animal Science And Technology Northwest A&f University:department Of Biological Engin
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Han Zhengsheng
Department Of Biological Engineering College Of Environment & Chemical Engineering Yanshan Unive
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Zhao Rui
Department Of Biological Engineering College Of Environment & Chemical Engineering Yanshan Unive
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Jiang Zhongliang
College Of Animal Science And Technology Northwest A&f University
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Li Jian
Department Of Applied Mathematics Northwestern Polytechnical University
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Li Qing
College of Animal Science and Technology, Northwest A&F University
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