Usefulness of Breath Test for Evaluating Pancreatic Exocrine Function Using N-Benzoyl-L-tyrosyl-1-^<13>C-L-alanine Sodium in Non-invasive and Conscious Rats(Analytical Biochemistry)
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概要
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N-Benzoyl-L-tyrosyl-1-^<13>C-L-alanine sodium is a dipeptide used for evaluating pancreatic exocrine function. The method of evaluation, however, is not yet satisfactory, especially in experimental animals. The relation between diabetes and pancreatic exocrine function also is not clear. Therefore, this study sought to establish a method for evaluating pancreatic exocrine function and to validate the method by determining non-invasively the effect of alloxan-induced diabetes in conscious rats. After fasting, rats were orally administered Racol containing N-benzoyl-L-tyrosyl-1-^<13>C-L-alanine sodium or 1-^<13>C-L-alanine and housed in desiccators. The expired air in the desiccator was collected in a breath-sampling bag using a tube and aspiration pump, and the level of ^<13>CO_2 in this air was measured using an infrared spectrometer at appropriate intervals over a 120 min period. The rate of ^<13>CO_2 excretion increased, peaked and then decreased in a dose-dependent manner. The maximum concentration and area under the curve of ^<13>CO_2 excretion significantly and positively correlated with the doses of N-benzoyl-L-tyrosyl-1-^<13>C-L-alanine sodium. In the rats made diabetes by the administration of alloxan, the level of expired ^<13>CO_2 air changed at significantly lower levels as compared with that of the control rats on day 3, although the level of expired ^<13>CO_2 air from 1-^<13>C-L-alanine was also markedly lower than that of the control rats. These results showed that pancreatic exocrine function can be evaluated using this breath test system and that alloxan-induced diabetes affects this function.
- 公益社団法人日本薬学会の論文
- 2008-05-01
著者
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MOGAMI Orie
Food Functionality Research Institute, Division of Research and Development, Meiji Dairies Corporati
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Mogami Orie
Food Science Institute Division Of Research And Development Meiji Dairies Corporation
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Uchida Masayuki
Food Functionality Research Institute Division Of Research And Development Meiji Dairies Corporation
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