35.ミヤコグサの就眠運動に関与する覚醒物質の探索(口頭発表)

概要

論文の詳細を見る
Most leguminous plants close their leaves in the evening, as if to sleep, and open them in the morning, called nyctinasty. The circadian rhythmic movements of their leaves are controlled by a pair of leaf-closing and -opening factors to vary the ratio between the two compounds. Five pairs of leaf-movement factors were identified from five legumes, respectively, and recent study revealed that each leaf-movement factor interacts its specific receptor. But, it seemed to be difficult to elucidate the detail of the signal transduction of nyctinasty, since these legumes have less genetic information. Here we reported to identify a novel leaf-opening factor from Lotus japonicus, possessing major advantages as a model legume for the study of genetic information. We screened the leaf-opening factor from extract of L. japonicus by a bioassay system using a leaf of L. japonicus. After solvent partition, the bioactive aqueous layer was purified by four steps of column chromatography and HPLC to give a pure active compound (136μg), which kept opening the leaf at 1 mg/L. The structure of the compound was identified as eucomic acid based on 800 MHz NMR with cold prove and MS/MS analyses. We synthesized eucomic acid, and its NMR spectrum and retention time of LC/MS analyses were identical to those of natural one. Furthermore, synthetic eucomic acid showed a significant biological activity at 10μM. We have thus confirmed that the leaf-opening factor of L. japonicus is eucomic acid.
植物化学調節学会の論文
2007-10-05

35.ミヤコグサの就眠運動に関与する覚醒物質の探索(口頭発表)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク