ELECTRON MICROSCOPIC AND IMMUNOHISTOCHEMICAL STUDY OF THE NEURONS AND THE AGRANULAR CELLS IN THE PARS TUBERALIS AND "TRANSITIONAL ZONE" OF THE PITUITARY GLAND

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In the regulatory system of the hypothalamo-hypophysial-gonadal axis, the localization and morphological characters of LHRH neurons have been studied by many researchers in the brain, pars tuberalis and anterior pituitary gland of rats. Kurita et al. (2004) and Sato et al. (2005 a, b) pointed out the possible significance of the "transitional zone" of the anterior pituitary as a hormonal-controlling system. The morphological and physiological significance of LHRH fibers in the basal layer of the pars tuberalis is still unclear. Ten intact 60-day-old male Wistar-Imamichi rats were used in the current study. Five animals were perfused with glutaraldehyde for electron microscopy, while the remained five animals were perfused with Bouin's solution without acetic acid at 4℃ for immunohistochemical analysis of LHRH and neurofilament (NF) protein. The glandular structure of the frontal region of the pars tuberalis consisted of agranular, folliculo-stellate cells. The NF-positive sites were observed in the rostral portion of the gland where none of LHRH-positive sites were located. Anteriorly in the portal vein segment (APV), vertically running LHRH-positive fivers became conspicuous in the brain. The NF-positive sites were clearly observed around the blood vessels and along the basal layer of the gland. It is notable that the LHRH- and NF-positive reactions were present at the floor of the pars tuberalis prior to finding them within the pars tuberalis, beginning at the APV and extending to the posterior portion of the portal vein segment (PPV). In the electron microscopic observations, bundles of endocrine and unmyelinated fibers separated the pars tuberalis from the brain, while bundles of endocrine and unmyelinated fibers were often observed at the basal layer of both the APV and PPV. The myelinated and unmyelinated fibers formed clusters among the pituitary cells at the "transitional zone" of the anterior lobe. The cytoarchitecture of the LHRH system is discussed relative to its control of finer endocrine regulation in the pituitary gland including circadian rhythm.
名古屋市立大学の論文
2007-01-31

ELECTRON MICROSCOPIC AND IMMUNOHISTOCHEMICAL STUDY OF THE NEURONS AND THE AGRANULAR CELLS IN THE PARS TUBERALIS AND "TRANSITIONAL ZONE" OF THE PITUITARY GLAND

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