Interspecific Electrofusion of Protoplasts between Aspergillus oryzae and Aspergillus sojae(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
An α-amylase hyper producer A. oryzae and a pro tease hyper producer A. sojae were electrofused to breed more excellent koji-molds. Fusion between the yellow-spored, pant mutant or the yellow-spored, met mutant of A. oryzae and the white-spored, bio mutant or the white-spored, ade mutant of A. sojae produced heterokaryons with a frequency of 2×10^<-5> per protoplast pair. The optimal parameters for the fusion of Aspergillm protoplasts were 1.1 MHz AC frequency, 400 V/cm AC voltage, 4kV/cm DC pulse voltage, 60μsec pulse duration, and 2 or 3 pulses. In this combination of the fusion-parents, all the fusants with stable green conidia formed an alkaline proteinase with the mobility of A. oryzae-type, and none of them formed any intermediate type. The productivity and molecular specificity of proteinases behaved independently from each other in the processes of fusion and haploidization.
- 社団法人日本農芸化学会の論文
- 1991-01-23
著者
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Uchida Koji
Graduate School Of Bioagricultural Sciences Nagoya University
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UCHIDA Kinji
Soy Sauce Research Laboratory, R & D Division, Kikkoman Corporation
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Uchida K
Soy Sauce Research Laboratory Kikkoman Corp.
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Uchida Kinji
Soy Sauce Research Laboratories Kikkoman Corp.
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USHIJIMA Shigeomi
Soy Sauce Research Laboratory, Kikkoman Corp.
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NAKADAI Tadanobu
Soy Sauce Research Laboratory, Kikkoman Corp.
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Nakadai T
Noda Inst. Scientific Research Noda Jpn
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Nakadai Tadanobu
Soy Sauce Research Laboratory Kikkoman Corp.
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Ushijima Shigeomi
Soy Sauce Research Laboratory Kikkoman Corp.
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