Solubilization of 4-Hydroxybenzoate-polyprenyltransferase from Cell Membrane of Pseudomonas putida and Its Properties(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
The enzyme of the first step in ubiquinone biosynthesis, 4-hydroxybenzoate-polyprenyltransferase was solubilized with 0.3% Triton X-100 from a membrane fraction of Pseudomonas putida IAM 1219. The enzyme had an optimum pH at 7.0-7.2 in 50 mM imidazole buffer and the apparent Km was 1.4×10^<-4>M for nonaprenylpyrophosphate and 0.54×10^<-5>M for 4-hydroxybenzoate. The enzyme required magnesium ion, and was completely inhibited by Cb^<2+>, Cu^<2+>, Ag^+, Hg^<2+> at 0.1 mM concentation, and was also strongly inhibited by the thiol reagents, 4-chloromercuribenzoate and N-ethylmaleimide, indicating that the transferase is a thiol enzyme. The enzyme was strongly activated by adding phospholipids extracted from the bacterial cells, of which phosphatidylethanolamine was most effective.
- 社団法人日本農芸化学会の論文
- 1991-09-23
著者
-
Uchida Koji
Graduate School Of Bioagricultural Sciences Nagoya University
-
Uchida K
Soy Sauce Research Laboratory Kikkoman Corp.
-
Uchida Kinya
Institute Of Applied Microbiology The University Of Tokyo
-
Kawahara Kazyyoshi
Institute Of Applied Microbiology The University Of Tokyo
-
KOIZUMI Naohisa
Institute of Applied Microbiology, The University of Tokyo
-
KAWAJI Haruhiko
Institute of Applied Microbiology, The University of Tokyo
-
Kawaji Haruhiko
Institute Of Applied Microbiology The University Of Tokyo
-
Koizumi Naohisa
Institute Of Applied Microbiology The University Of Tokyo
関連論文
- Inhibitory Effect of Histamine H_3-Receptor Antagonist Cimetidine against Copper(II)/Ascorbate-mediated Protein Damage(Food & Nutrition)
- Stimulation of Iron/Ascorbate-dependent Lipid Peroxidation by Histamine(Food & Nutrition)
- Partial Purification and Characterization of 4-Hydroxybenzoate-polyprenyltransferase in Ubiquinone Biosynthesis of Pseudomonas putida(Biological Chemistry)
- Solubilization of 4-Hydroxybenzoate-polyprenyltransferase from Cell Membrane of Pseudomonas putida and Its Properties(Biological Chemistry)
- Isolation and Partial Characterization of a Cerulenin-sensitive Mutant of Pseudomonas aeruginosa
- Modulatory effects of black tea polyphenols on oxidant-antioxidant profile and expression of proliferation, apoptosis, and angiogenesis-associated proteins in the rat forestomach carcinogenesis model
- P-494 Polymer-Assisted Solution-phase Synthesis of 15-Deoxy-Δ^-PGJ_2 Derivatives and their Neurite Outgrowth-Promoting Activity
- Prostaglandins from a Zoanthid : Paclitaxel-Like Neurite-Degenerating and Microtubule-Stabilizating Activities
- 16(I-2) Novel Saponins from the Okinawan Starfish Linckia laevigata : Structure Determination, Neuritogenic Activity, and Mechanism of Action
- Interspecific Electrofusion of Protoplasts between Aspergillus oryzae and Aspergillus sojae(Microbiology & Fermentation Industry)
- Further Evidence on the Interspecific Protoplast Fusion between Aspergillus oryzae and Aspergillus sojae and Subsequent Haploidization, with Special Reference to Their Production of Some Hydrolyzing Enzymes(Microbiology & Fermentation Industry)
- Breeding of New Koji-molds through Interspecific Hybridization between Aspergillus oryzae and Aspergillus sojae by Protoplast Fusion(Microbiology & Fermentation Industry)
- Oxidative Depolymerization of Polysaccharides Induced by the Ascorbic Acid-Copper Ion Systems(Food & Nutrition)
- On Silverman's estimate of regulators
- Regular Representations of Codes and Duality
- A Simple Method for Isolation of Lipopolysaccharides from Pseudomonas aeruginosa and Some Other Bacterial Strains(Microbiology & Fermentation Industry)