A Simple Method for Isolation of Lipopolysaccharides from Pseudomonas aeruginosa and Some Other Bacterial Strains(Microbiology & Fermentation Industry)

スポンサーリンク

概要

• 論文の詳細を見る

• A simple procedure for the isolation of bacterial lipopolysaccharides was developed. The method consists of the following three steps; recovery of lipopolysaccharides in an insoluble form together with denatured proteins in a hot MgCl_2-Triton X-100 solution (step 1), solubilization with EDTA-Triton X-100 (step 2) and precipitation with MgCl_2 (step 3). A good yield of lipopolysaccharides was obtained with most of the Pseudomonas strains tested. Although the yield was lower with strains belonging to Salmonella and Escherichia, irrespective of whether they are smooth-type or rough-type strains, the amounts obtained from 100 mg of cells as dry weight were sufficient for detailed chemical characterization. The gel-electrophoretic profiles of the lipopolysaccharides prepared by the present method were essentially the same as those of the lipopolysaccharides prepared by the conventional phenol-water method. The method allowed us to prepare lipopolysaccharides from a large number of bacterial strains in one day.

• 社団法人日本農芸化学会の論文
• 1987-11-23

著者

• Uchida Kinya

  Institute Of Applied Microbiology The University Of Tokyo

• Mizushima Shoji

  Institute Of Applied Microbiology The University Of Tokyo

関連論文

• Partial Purification and Characterization of 4-Hydroxybenzoate-polyprenyltransferase in Ubiquinone Biosynthesis of Pseudomonas putida(Biological Chemistry)
• Solubilization of 4-Hydroxybenzoate-polyprenyltransferase from Cell Membrane of Pseudomonas putida and Its Properties(Biological Chemistry)
• Large-scale Production of Membrane Proteins Fused to a Truncated SecA in Escherichia coli
• A Simple Method for Isolation of Lipopolysaccharides from Pseudomonas aeruginosa and Some Other Bacterial Strains(Microbiology & Fermentation Industry)

スポンサーリンク