Purification and Reaction of a New Enzyme, Nucleoside Oxidase(Biological Chemistry)
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概要
- 論文の詳細を見る
Nucleoside oxidase, a novel nucleoside oxidizing enzyme has been purified from a crude extract of Pseudomonas maltophilia LB-86 by a ammonium sulfate fractionation, heat treatment, column chromatography on DEAE-Toyopearl, and gel filtration twice on Sephacryl S-200. The overall purification was approximately 60-fold with a yield of 35%. The purified enzyme gave a single protein band on acrylamide gel electrophoresis. Reaction products from inosine were identified as inosine-5'-aldehyde and inosine-5'-carboxylic acid. The enzyme catalyzed the oxidation of inosine to inosine-5'-carboxylic acid via inosine-5'-aldehyde using molecular oxygen as a primary electron acceptor with no formation of hydrogen peroxide.
- 社団法人日本農芸化学会の論文
- 1989-06-23
著者
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Hoshino M
Otsuka Foods Co. Ltd. Shiga Jpn
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Hoshino Masami
Biwako Research Institute Otsuka Foods Co. Ltd.
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ISONO Yoshikazu
Biwako Research Institute, Otsuka Foods Co., Ltd.
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SUDO Tomoko
Biwako Research Laboratory, Otsuka Foods Co., Ltd.
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Sudo Tomoko
Biwako Research Laboratory Otsuka Foods Co. Ltd.
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Isono Y
Otsuka Foods Co. Ltd. Shiga Jpn
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Isono Yoshikazu
Biwako Research Institute Otsuka Foods Co. Ltd.
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