A New Kinetic Method for Assaying Serum 5'-Nucleotidase Using Nucleoside Oxidase(Analytical Chemistry)
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概要
- 論文の詳細を見る
A sensitive kinetic determination of serum 5'-nucleotidase activity is described. Nucleoside oxidase catalyzes the oxidative coupling reaction of phenolic compounds and 4-aminoantipyrine to form colored substances in proportion of the amount of nucleoside. The present method relies on this phenomenon. Human serum 5'-nucleotidase was assayed using 5'-AMP as a substrate. When adenosine liberated was oxidized by nucleoside oxidase in the presence of phenolic compounds and 4-aminoantipyrine, a colored substance was formed at the same time. The rate of colored substance formation was proportional to the 5'-nucIeotidase activity. The proposed method shows a low variation and a good correlation with the reference method. 3'-Nucleotidase was also assayed in a similar manner.
- 社団法人日本農芸化学会の論文
- 1989-10-23
著者
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Hoshino Masami
Biwako Research Institute Otsuka Foods Co. Ltd.
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ISONO Yoshikazu
Biwako Research Institute, Otsuka Foods Co., Ltd.
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TSUJIMOTO Hiroko
Biwako Research Institute, Otsuka Foods Co., Ltd.
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Isono Yoshikazu
Biwako Research Institute Otsuka Foods Co. Ltd.
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Tsujimoto Hiroko
Biwako Research Institute Otsuka Foods Co. Ltd.
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