Properties of a New Enzyme, Nucleoside Oxidase, from Pseudomonas maltophilia LB-86(Biological Chemistry)
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概要
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Nucleoside oxidase purified from Pseudomonas maltophilia LB-86 had mol. wt. = 130.000 and was composed of one each of four non-identical subunits: subunit α, 76,000; subunit β, 33,000; subunit γ, 18,000; subunit δ, 14,000. The enzyme contains 1 mol of covalently bound FAD, 2 g atoms of non-heme iron, 2 mol of labile sulfides, and 1 mol of heme per mol enzyme protein. The absorption spectrum of nucleoside oxidase had maxima 278 and 390 nm, and shoulders at 343 and 450 nm, The enzyme catalyzes the oxidation of various nucleosides, and the Km value for inosine was 4.4×10^<-5> M. The enzyme was most active at pH 5〜6, and was most stable between pH 5.0〜6.0 and at temperatures below 600℃. The activity was strongly inhibited by N-bromosuccinimide and potassium cyanide.
- 社団法人日本農芸化学会の論文
- 1989-06-23
著者
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Hoshino M
Otsuka Foods Co. Ltd. Shiga Jpn
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Hoshino Masami
Biwako Research Institute Otsuka Foods Co. Ltd.
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ISONO Yoshikazu
Biwako Research Institute, Otsuka Foods Co., Ltd.
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SUDO Tomoko
Biwako Research Laboratory, Otsuka Foods Co., Ltd.
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Sudo Tomoko
Biwako Research Laboratory Otsuka Foods Co. Ltd.
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Isono Y
Otsuka Foods Co. Ltd. Shiga Jpn
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Isono Yoshikazu
Biwako Research Institute Otsuka Foods Co. Ltd.
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