キサンチン酸化によるミノールの化学発光

概要

論文の詳細を見る
Since reactive oxygen species have been postulated to underlie the pathogenesis of various diseases, measurement of luminol chemiluminescence (LCL) has recently been used to quantitate active oxygen production. However, the precise mechanism of LCL and active oxygen species which react with luminol have not been elucidated. Oxidation of xanthine by xanthine oxidase (X-XO system) is one of the main superoxide generating reactions in vivo, in which the superoxide is changed to several reactive oxygen species, such as hydrogen peroxide, hypochlorite, singlet oxygen and hydroxy radicals by enzymatic or nonenzymatic reactions. In this experiment, we clarified the fact that LCL by X-XO system is mainly dependent on uric acid-inhibitable hydroxy radicals which are produced from superoxide radicals and hydrogen peroxide by chemical reaction.
高知大学の論文
1990-12-10