好中球の刺激に伴う活性酸素生成とその刺激物特異性について

概要

論文の詳細を見る
Upon interaction with soluble or particulate matter, neutrophils produce active oxygen species. Since luminol-dependent chemiluminescence (CL) in neutrophils can blocked by azide, an inhibitor of myeloperoxidase activity, it has been believed to reflect the peroxidasecatalyzed reaction. The sensitivity of CL to superoxide dismutase (SOD) was very high when the cells were stimulated with formylmethionyl-1eucyl-phenylalane (FMLP) but not by phorbol myristate acetate (PMA) or opsonized zymosan (OZ). Luminol-dependent CL can be blocked by azide when neutrophils were stimulated by OZ or PMA. In contrast, the inhibitory action of azide was not observed in the case of stimulation by FMLP. These results were also supported to the measurement of H202 in suspending medium : when cells were stimulated by arachidonic acid, PMA and OZ, hydrogen peroxide production by neutrophils were increased by addition of superoxide dismutase (SOD) or azide. Furthermore, stimulation-dependent CL, especially by FMLP, was increased by vanadete. In this case, vanadate was reacting with O^∸_2 to yield an oxidant capable of initiating a free radical chain oxidation of NADPH. Thus, the oxidant species and the enzymatic pathway of the phagocytic cells follwing of their respiratory burst by various stimuli are distinctly different from one stimuli to another.
高知大学の論文
1989-12-10