口腔内多形核白血球の刺激応答性

概要

論文の詳細を見る
Salivary neutrophils (SPMN) were isolated from the oral cavity and stimulation-coupled responses were examined. From the morphological study and content of neutrophil specific 39kDa protein, these cells seems to be similar with those of peritoneal neutrophils. Most of the neutrophils were young stage cells. Stimulation-coupled responses of these cells were observed in superoxide (O^∸_2) generation, Iuminol chemiluminescence (LCL) response, membrane deporalization and changes in intracellular calcium ions. However, strong O^∸_2 generation was observed without addition of any stimulants. This LCL was inhibited by azide but not by uric acid or superoxide dismutase (SOD). The endogenous LCL decreased from the time after isolation from the oral cavity accompanied by the appearance of FMLP-coupled response. The rate of active oxygen generation by PMA was similar with that of peritoneal neutrophils, but opsonized zymosan (OZ) induced LCL was lower than that of peripheral neutrophils. Furthermore, the activity of active oxygen generation was maintained for a long period (more than 10 hours). From these evidences, it is concluded that the salivary neutrophils have the specific characteristics of stimulation-coupled responses, and play an important role in the defense mechanism of the oral cavity.
高知大学の論文
1990-12-10