Growth Factor Array Fabrication Using a Color Ink Jet Printer(Cell Biology)

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概要

• 論文の詳細を見る

• We have developed a novel method for growth factor analysis using a commercial color ink jet printer to fabricate substrata patterned with growth factors. We prepared substrata with insulin printed in a simple pattern or containing multiple areas of varying quantities of printed insulin. When we cultured the mouse myoblast cell line, C2C12, on the insulin-patterned substrata, the cells were grown in the same pattern with the insulin-printed pattern. Cell culture with the latter substrata demonstrated that quantity control of insulin deposition by a color ink jet printer is possible. For further applications, we developed substrata with insulin-like growth factor-l (IGF-I) and basic fibroblast growth factor (bFGF) spotted in 16 different areas in varying combinations and concentrations (growth factor array). With this growth factor array, C2C12 cells were cultured, and the onset of muscle cell differentiation was monitored for the expression of the myogenic regulator myogenin. The ratio of cells expressing myogenin varied with the doses of IGF-I and bFGF in the sections, demonstrating a feasibility of growth factor array fabrication by a color ink jet printer. Since a printer manipulates several colors, this method can be easily applied to multivariate analyses of growth factors and attachment factors affecting cell growth and differentiation. This method may provide a powerful tool for cell biology and tissue engineering, especially for stem cell research in investigating unknown conditions for differentiation.

• 社団法人日本動物学会の論文
• 2003-04-25

著者

• Watanabe K

  Tohoku Univ. Sendai Jpn

• MATSUDA Ryoichi

  Department of Life Sciences, The University of Tokyo

• Watanabe Kohei

  Canon Inc.

• Watanabe Kenji

  Graduate School Of Life Science University Of Hyogo

• Watanabe Kazutada

  Department Of Experimental Biology Tokyo Metropolitan Institute Of Gerontology

• Miyazaki T

  Canon Inc.

• Miyazaki Takeshi

  Canon Inc.

• Watanabe Kohei

  Department of Biological Science, Graduate School of Science, the University of Tokyo

• Matsuda R.

  Department Of Biological Science Graduate School Of Science The University Of Tokyo:department Of Li

• Matsuda Ryoichi

  Department Of Biological Sciences Graduate School Science The University Of Tokyo Meguro-ku:departme

• Matsuda Ryoichi

  Department Of Life Science The University Of Tokyo

• Matsuda Ryoichi

  Department Of Life Science University Of Tokyo

• Matsuda Ryoichi

  Department of Applied Chemistry Faculty of Engineering Tokushima University

• Watanabe Kohei

  Department of Applied Chemistry, Faculty of Engineering, Keio University

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