Identification of two distinct muscles in the planarian Dugesia japonica by their expression of myosin heavy chain genes
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概要
- 論文の詳細を見る
Ultrastructural and physiological studies have shown that planarian muscles have some characteristics of smooth and some characteristics of striated muscles. To characterize planarian muscles, we isolated two myosin heavy chain genes (DjMHC-A and DjMHC-B) from a planarian, Dugesia japonica, by immunological screening, and analyzed their structures and spatial expression patterns. Structural analysis indicated that both MHC genes are striated-muscle-type myosin genes, although planarian muscles do not have any striation. In situ RNA hybridization showed that expression of the two myosin genes is spatially strictly segregated. DjMHC-A was expressed in pharynx muscles, pharynx cavity muscles, muscles surrounding the intestinal ducts, a subpopulation of body-wall muscles and several muscle cells in the mesenchymal region around the base of the pharynx. DjMHC-B was expressed in body-wall muscles (including circular, diagonal and longitudinal muscles), vertical muscles and horizontally oriented muscles. Double staining with DjMHC-A and -B probes clearly demonstrated that expression of the DjMHC-A and -B genes do not occur in the same cell. During regeneration, the number of cells positive for expression of each gene increased in the blastema region, suggesting that both types of muscle may be involved in blastema formation. DjMHC-B-positive cells disappeared from the body-wall muscle layer in the pharynx-cavity-forming region, whereas DjMHC-A-positive cells were markedly accumulated there, suggesting that the two types of muscle in the body wall layer may have distinct functions. These results indicate that planarians have at least two types of muscle that express striated-muscle-type MHC genes, but do not form striation.
- 社団法人日本動物学会の論文
- 1998-12-15
著者
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KOBAYASHI Shuji
Okayama University of Science
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Agata K
Dep. Of Biophysics Graduate School Of Sci. Kyoto Univ.
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Agata Kiyokazu
Lab. Mol. Dev. Biol. Grad. Sch. Sci. Kyoto Univ.
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Agata Kiyokazu
Genome Resource And Analysis Subunit Riken Center For Developmental Biology
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Watanabe Kohei
Canon Inc.
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Agata Kiyokazu
Dep. Of Biophysics Graduate School Of Sci. Kyoto Univ.
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Agata Kiyokazu
Laboratory For Evolutionary Regeneration Center For Developmental Biology Riken
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Watanabe Kenji
Graduate School Of Life Science University Of Hyogo
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Orii Hidefumi
Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute
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Watanabe Kenji
Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute
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Kobayashi Chiyoko
Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute
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Agata Kiyokazu
Group For Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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Kobayashi Seitaro
Laboratory of Regeneration Biology, Department of Life Science, Faculty of Science, Himeji Institute
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Orii H
Graduate School Of Life Science University Of Hyogo
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Orii Hidefumi
Department Of Life Science Graduate School Of Science Himeji Institute Of Technology
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Orii Hidefumi
Laboratory Of Regeneration Biology Department Of Life Science Graduate School Of Science Himeji Inst
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Watanabe Kazutada
Department Of Experimental Biology Tokyo Metropolitan Institute Of Gerontology
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Kobayashi Shuji
Department Of Biosphere-geosphere System Science Faculty Of Informatics Okayama University Of Scienc
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Kobayashi Seitaro
Okazaki Institute For Integrative Bioscience National Institute For Basic Biology
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小林 秀司
岡山理科大学総合情報学部生物地球システム学科
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Kobayashi C
Laboratory For Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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Kobayashi Chiyoko
Laboratory For Evolutionary Regeneration Center For Developmental Biology Riken Kobe
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小林 秀司
Okayama University Of Science
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Kobayashi Seitaro
Laboratory Of Regeneration Biology Department Of Life Science Faculty Of Science Himeji Institute Of
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Watanabe Kenji
Laboratory Of Regeneration Biology Department Of Life Science Graduate School Of Science Himeji Inst
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Watanabe Kenji
Laboratory Of Food Material Engineering Faculty Of Agriculture Gifu University
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Kobayashi Chiyoko
Laboratory for Evolutionary Regeneration Biology, Center for Developmental Biology, RIKEN Kobe
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