Morphological and functional recovery of the planarian photosensing system during head regeneration
スポンサーリンク
概要
- 論文の詳細を見る
When exposed to light, planarians display a distinctive light avoidance behavior known as negative phototaxis. Such behavior is temporarily suppressed when animals are decapitated, and it is restored once the animals regenerate their heads. Head regeneration and the simple but reproducible phototactic response of planarians provides an opportunity to study the association between neuronal differentiation and the establishment of behavior in a simple, experimentally tractable metazoan. We have devised a phototaxis assay system to analyze light response recovery during head regeneration and determined that light evasion is markedly re-established 5 days after amputation. Immunohistological and in situ hybridization studies indicate that the photoreceptors and optic nerve connections to the brain begin by the fourth day of cephalic regeneration. To experimentally manipulate the light response recovery, we performed gene knockdown analysis using RNA interference (RNAi) on two genes (1020HH and eye53) previously reported to be expressed at 5 days after amputation and in the dorso-medial region of the brain (where the optic nerves project). Although RNAi failed to produce morphological defects in either the brain or the visual neurons, the recovery of the phototactic response normally observed in 5-day regenerates was significantly suppressed. The data suggest that 1020HH and eye53 may be involved in the functional recovery and maintenance of the visual system, and that the phototaxis assay presented here can be used to reliably quantify the negative phototactic behavior of planarians.
- 社団法人日本動物学会の論文
- 2004-03-25
著者
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Agata K
Dep. Of Biophysics Graduate School Of Sci. Kyoto Univ.
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Agata Kiyokazu
Lab. Mol. Dev. Biol. Grad. Sch. Sci. Kyoto Univ.
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Agata Kiyokazu
理化学研究所発生再生科学総合研究センター
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Agata Kiyokazu
Genome Resource And Analysis Subunit Riken Center For Developmental Biology
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Agata Kiyokazu
Dep. Of Biophysics Graduate School Of Sci. Kyoto Univ.
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Agata Kiyokazu
Groupfor Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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Agata Kiyokazu
Laboratory For Evolutionary Regeneration Center For Developmental Biology Riken
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Agata Kiyokazu
Riken Cdb Evo.
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Agata Kiyokazu
Group For Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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Inoue Takeshi
Faculty of Biomolecular Science, Okayama University Graduate School of Natural Science and Technolog
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Kumamoto Hiroshi
Department of Biology, Faculty of Science, Okayama University
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Okamoto Keiji
Group for Evolutionary Regeneration Biology, RIKEN Center for Developmental Biology
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Umesono Yoshihiko
Group for Evolutionary Regeneration Biology, RIKEN Center for Developmental Biology
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Sakai Masaki
Department of Biology, Faculty of Science, Okayama University
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Alvarado Alejandro
Department of Neurobiology and Anatomy School of Medicine, University of Utah
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Inoue T
Evolutionary Regeneration Group Center For Developmental Biology Riken:kan Research Institute
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Alvarado Alejandro
Department Of Neurobiology And Anatomy School Of Medicine University Of Utah
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Inoue T
Group For Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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Agata Kiyokazu
Lab. For Mol. Dev. Biol. Dept. Of Biophys. Grad. Sch. Of Sci. Kyoto Univ.
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Okamoto Keiji
Group For Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe:department O
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Okamoto Keiji
Group For Evolutionary Regeneration Biology Riken Center For Developmental Biology:department Of Bio
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Sakai Masaki
Department Of Biology Faculty Of Science Okayama University
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Kumamoto Hiroshi
Department Of Biology Faculty Of Science Okayama University
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Umesono Y
Group For Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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Umesono Yoshihiko
Group for Evolutionary Regeneration Biology, Center for Developmental Biology, RIKEN Kobe
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