Cultivation and characterization of planarian neuronal cells isolated by fluorescence activated cell sorting (FACS)
スポンサーリンク
概要
- 論文の詳細を見る
Studies using molecular markers have revealed that planarians possess a highly organized brain. Here we separated brain neurons from dissociated planarian head cells by fluorescence activated cell sorting (FACS), and characterized them by single cell PCR analysis and cell culture. Dissociated cells were labeled with three different fluorescent dyes, Hoechst 33258, Merocyanine 540, and Propidium Iodide (PI), and fractioned by FACS. Interestingly, we have succeeded in identifying a cell fraction specific to the head, which we have named the head-abundant cell fraction (HAC). Most of the HAC expressed neuron-specific genes and proteins. When they were cultured in vitro, they showed an ability to extend neurites on several types of extracellular matrices (ECMs), and, depending on the ECM type used, presented a high level of plasticity in morphology and gene expression.
- 社団法人日本動物学会の論文
- 2002-11-25
著者
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Agata K
Dep. Of Biophysics Graduate School Of Sci. Kyoto Univ.
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Agata Kiyokazu
Lab. Mol. Dev. Biol. Grad. Sch. Sci. Kyoto Univ.
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Agata Kiyokazu
Riken Center For Developmental Biology
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Agata Kiyokazu
Evolutionary Regeneration Group Center For Developmental Biology Riken
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Agata Kiyokazu
理化学研究所発生再生科学総合研究センター
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Agata Kiyokazu
Genome Resource And Analysis Subunit Riken Center For Developmental Biology
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KAGAWA Hiroaki
Department of Biology, Faculty of Science, Okayama University
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Kagawa H
Miyazaki Univ. Miyazaki Jpn
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Kagawa Hiroaki
Department Of Biology Faculty Of Science Okayama University
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Agata Kiyokazu
Dep. Of Biophysics Graduate School Of Sci. Kyoto Univ.
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Agata Kiyokazu
Laboratory For Evolutionary Regeneration Center For Developmental Biology Riken
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Agata Kiyokazu
Riken Cdb Evo.
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Agata Kiyokazu
Evolutionary Regeneration Biology Group Riken Center For Developmental Biology
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Asami Maki
Riken Center For Developmental Biology
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Agata Kiyokazu
Group For Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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HAYASHI Tetsutaro
Evolutionary Regeneration Biology Group, RIKEN Center for Developmental Biology
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HAYASHI Tetsutaro
RIKEN Center for Developmental Biology
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Asami Maki
Department of Biology, Faculty of Science, Okayama University
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Nakatsuka Tsuyoshi
Department of Life Science, Faculty of Science, Himeji Institute of Technology
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Kou Kenji
Department of Biology, Faculty of Science, University of Okayama
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Kou K
Department Of Biology Faculty Of Science University Of Okayama
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Agata Kiyokazu
Lab. For Mol. Dev. Biol. Dept. Of Biophys. Grad. Sch. Of Sci. Kyoto Univ.
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Hayashi T
Riken Center For Developmental Biology
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Hayashi Tetsutaro
Evolutionary Regeneration Biology Group Riken Center For Developmental Biology
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Nakatsuka Tsuyoshi
Department Of Life Science Faculty Of Science Himeji Institute Of Technology
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Kagawa H
Faculty Of Aguriculture University Of Miyazaki
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