Isolation of Ef silicatein and Ef lectin as molecular markers for sclerocytes and cells involved in innate immunity in the freshwater sponge Ephydatia fluviatilis
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概要
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Sponges (phylum Porifera) have remarkable regenerative and reconstitutive abilities and represent evolutionarily the oldest metazoans. To investigate sponge stem cell differentiation, we have focused on the asexual reproductive system in the freshwater sponge Ephydatia fluviatilis. During germination, thousands of stem cells proliferate and differentiate to form a fully functional sponge. As an initial step of our investigation of stem cell (archeocyte) differentiation, we isolated molecular markers for two differentiated cell types: spicule-making sclerocyte cells, and cells involved in innate immunity. Sclerocyte lineage-specific Ef silicatein shares 45% to 62% identity with other sponge silicateins. As in situ hybridization of Ef silicatein specifically detects archeocytes possibly committed to sclerocytes, as well as sclerocytes with an immature or mature spicule, therefore covering all the developmental stages, we conclude that Ef silicatein is a suitable sclerocyte lineage marker. Ef lectin, a marker for the cell type involved in innate immunity, shares 59% to 65% identity with the marine sponge Suberites domuncula galactose-binding protein (Sd GBP) and horseshoe crab Tachypleus tridentatus tachylectin1/lectinL6. Since Scl GBP and tachylectin1 are known to bind to bacterial lipopolysaccharides and inhibit the growth of bacteria, Ef lectin may have a similar function and be expressed in a specialized type of cell involved in defense against invading bacteria. Ef lectin mRNA and protein are not expressed in early stages of development, but are detected in late stages. Therefore, Ef lectin may be specifically expressed in differentiating and/or differentiated cells. We suggest Ef lectin as a marker for cells that assume innate immunity in freshwater sponges.
- 社団法人日本動物学会の論文
- 2005-10-25
著者
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Agata Kiyokazu
Groupfor Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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Agata Kiyokazu
Group For Evolutionary Regeneration Biology Center For Developmental Biology Riken Kobe
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MIYATA Takashi
Department of Iron and Steel Engineering, Nagoya University
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Iwabe Naoyuki
Department Of Biophysics Faculty Of Science Kyoto University
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Funayama Noriko
Group for Evolutionary Regeneration Biology, Center of Developmental Biology, RIKEN Kobe
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Nakatsukasa Mikiko
Group for Evolutionary Regeneration Biology, Center of Developmental Biology, RIKEN Kobe
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Kuraku Shigehiro
Laboratory for Evolutionary Morphology, Center for Developmental Biology, RIKEN Kobe
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Takechi Katsuaki
Group for Evolutionary Regeneration Biology, Center for Developmental Biologe, RIKEN Kobe
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Dohi Mikako
Group for Evolutionary Regeneration Biologe, Center for Developmental Biology, RIKEN Kobe
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Kuraku Shigehiro
Univ. Konstanz Konstanz Deu
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Kuraku Shigehiro
Laboratory For Evolutionary Morphology Riken Cdb
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Takechi Katsuaki
Group For Evolutionary Regeneration Biology Center For Developmental Biologe Riken Kobe
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Miyata Takashi
Department Of Chemistry And Materials Engineering Kansai University
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Dohi Mikako
Group For Evolutionary Regeneration Biologe Center For Developmental Biology Riken Kobe
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Funayama Noriko
Group For Evolutionary Regeneration Biology Center Of Developmental Biology Riken Kobe
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Miyata Takashi
Department Of Biophysics Graduate School Of Sciecne Kyoto University
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Miyata Takashi
Department Of Biophysics Faculty Of Science Kyoto University
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Nakatsukasa Mikiko
Group For Evolutionary Regeneration Biology Center Of Developmental Biology Riken Kobe
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Iwabe Naoyuki
Laboratory of Animal Cytogenetics, Division of Genome Dynamics, Creative Research Initiative "Sousei", Hokkaido University
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MIYATA Takashi
Department of Applied Physics, Tokai University
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IWABE Naoyuki
Department of Biology, Faculty of Science, Kyushu University
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