Comparative Study on Major Bioactive Components in Natural, Artificial and in-Vitro Cultured Calculus Bovis
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概要
- 論文の詳細を見る
Major bioactive components in various Calculus Bovis, including natural, artificial and in-vitro cultured Calculus Bovis, were comparatively studied. An approach of high-performance liquid chromatography coupled with ultraviolet and evaporative light scattering detections (HPLC/UV/ELSD) was established to simultaneously determinate six bioactive components thereof, including five bile acids (cholic acid, deoxycholic acid, ursodeoxycholic, chenodeoxycholic acid, hyodeoxycholic acid) and bilirubin. ELSD and UV detector were applied to detect bile acids and bilirubin respectively. The assay was performed on a C_<18> column with water-acetonitrile gradient elution and the investigated constituents were authenticated by comparing retention times and mass spectra with those of reference compounds. The proposed method was applied to analyze twenty-one Calculus Bovis extraction samples, and produced data with acceptable linearity, precision, repeatability and accuracy. The result indicated the variations among Calculus Bovis samples under different developmental conditions. Artificial and in-vitro cultured Calculus Bovis, especially in-vitro cultured ones, which contain total bioactive constituents no less than natural products and have the best batch-to-batch uniformity, suffice to be used as substitutes of natural Calculus Bovis.
- 公益社団法人日本薬学会の論文
- 2007-01-01
著者
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Zhang Wei-Dong
School of Pharmacy, Shanghai Jiao Tong University
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LIU Run-Hui
Department of Phytochemistry, School of Pharmacy, Second Military Medical University
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Zhang Wei-dong
Department Of Phytochemistry School Of Pharmacy Second Military Medical University:school Of Pharmac
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Wu Yan-wen
School Of Pharmacy Shanghai Jiaotong University
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Liu Run-hui
Dep. Of Phytochemistry School Of Pharmacy Second Military Medical Univ.
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Yan Shi-kai
School Of Pharmacy Shanghai Jiao Tong Univ.
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Yan Shi-kai
School Of Pharmacy Shanghai Jiaotong University
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Zhang Wei-dong
School Of Pharmacy Shanghai Jiao Tong Univ.
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LIU Run-Hui
School of Pharmacy, Second Military Medical University
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