Simvastatin, Cholesterol-lowering Drug, as a New Therapeutic Agent for Periodontal Regeneration(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
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概要
- 論文の詳細を見る
In 1999, Mundy et al. has indicated that statins, drugs widely used for lowering serum cholesterol, stimulate bone formation in rodents and increase bone morphogenetic protein (BMP)-2 expression in vitro. However, the other growth factors except for BMP-2 and the effects of simvastatin, one of statins, on periodontal tissues have not been shown yet. The purpose of this study was to investigate the possibility of statins as a new effective agent for periodontal regeneration using luciferase reporter gene assay and reverse transcription-polymerase chain reaction (RT-PCR), and the alkaline phosphatase (ALP) activity in human periodontal ligament (HPDL) cells. The results showed that simvastatin induced not only BMP-2 but also transforming growth factor-β1 (TGF-β1) in HPDL cells and human osteoblastic cells (NHOst). To examine whether the TGF-a1 activity was related with productions of the cholesterol biosynthetic pathway, HPDL cells were treated with simvastatin in the presence or absence of mevalonic acid. The TGF-β1 induction was caused by the inhibition of cholesterol biosynthetic pathway. The differentiation study showed that TGF-β1 increased the ALP activity, but BMP-2 decreased the ALP activity in HPDL cells, even in the presence of TGF-β1. The effect of simvastatin is similar to that for BMP-2 with TGF-β1. The above in vitro findings suggest that simvastatin may be effective for periodontal regeneration as new therapeutic agents to induce the regenerative factors such as TGF-β1 and BMP-2.
- 硬組織再生生物学会の論文
著者
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Arai Takashi
Department Of Pathology Tokyo Medical Dental University
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GOMI Kazuhiro
Department of Periodontics and Endodontics, Tsurumi University School of Dental Medicine
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Gomi Kazuhiro
Department Of Periodontics And Endodontics School Of Dental Medicine Tsurumi University
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SUZUKI Shinichi
Department of Cardiovascular Surgery, Yokohama City University School of Medicine, Cardiovascular Ce
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NAGANO Takatoshi
Department of Periodontics and Endodontics, School of Dental Medicine, Tsurumi University
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OIDA Shinichiro
Department of Biochemistry, School of Dental Medicine, Tsurumi University
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FUKAE Makoto
Department of Biochemistry, School of Dental Medicine, Tsurumi University
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Karakida Takeo
Department of Biochemistry, School of Dentistry, Tsurumi University
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Oida Shinichiro
Department Of Biochemistry Faculty Of Dentistry Tokyo Medical And Dental University
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Oida Shinichiro
Department Of Biochemistry Tsurumi University School Of Dental Medicine
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Fukae Makoto
Department Of Biochemistry School Of Dental Medicine Tsurumi University
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Karakida Takeo
Department Of Biochemistry School Of Dentistry Tsurumi University
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Arai Takashi
Department Of Periodontics And Endodontics Tsurumi University School Of Dental Medicine
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Nagano Takatoshi
Department Of Mechanical Science And System Engineering School Of Science And Engineering Meiji Univ
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Nagano Takatoshi
Department Of Periodontics And Endodontics School Of Dental Medicine Tsurumi University
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Arai Takashi
Department Of Periodontics And Endodontics School Of Dental Medicine Tsurumi University
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Arai Takashi
Department Of Applied Biological Science Faculty Of Science And Technology Tokyo University Of Scien
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Arai Takashi
Department Of Applied Biological Science Faculty Of Science And Technology Science University Of Tok
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Suzuki Shinichi
Department Of Periodontics And Endodontics School Of Dental Medicine Tsurumi University
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Suzuki Shinichi
Department Of Aeronautical Engineering Nagoya University
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Oida Shinichiro
Department of Biochemistry, Tsurumi University School of Dental Medicine
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NAGANO Takatoshi
Department of Mechanical Science and System Engineering (MESSE), School of Science and Engineering, Meiji University
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ARAI TAKASHI
Department of Analytic Human Pathology, Internal Medicine, Nippon Medical School
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