Biological Activities of Bone Extract Containing TGF-β Superfamily Proteins

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Bone matrix contains many TGF-β superfamily proteins. The biological activity of these proteins are stable after decalcification with 0.6 M HCI and extraction with 4M guanidine-HCl. The extract can induce ectopic endochondral bone fomration in vivo. In the in vitro culture system, the same extract can inhibit cell growth in a mouse osteogenic cell line (MC3T3-E1) and a human squamous cell carcinoma cell line (HSC-4), and induce fibronectin production in HSC-4 and alkaline phosphatase in a mouse bone marrow stromal cell line (ST2). In this study, we purified the factors according to biological activities, and identified the growth and differentiation factor for each biological reaction, and confirmed the reciprocal reaction using the recombinant proteins. The results indicated that the ectopic bone formation was induced by BMP, a member of the TGF-β superfamily, and that growth inhibition and fibronectin induction in cultured cells depended on TGF-β, and that alkaline phosphatase induction by recombinant BMP-2 was strongly inhibited by recombinant TGF-β1 and the guanidine-HCI extract from bone matrix containing TGF-β.
硬組織再生生物学会の論文