硬組織の病理組織標本作成法

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Technical notes about calcified hard tissues including tooth and bone were described for new comers such as postgraduate students and young researchers. The macroscopic examinations are essential procedures before histological examinations. Especially, the examination by means of soft x-ray might provide useful findings about calcified hard tissue specimens. Decalcified method such as Plank-Rychlo quick decalcified procedure is commonly used for basic histomorphological examination using acids. Although the method might result decrease of Hematoxyline staining and over-staining of Eosin, it would be avoid by the decalcification under lower temperature in a refrigerator. The difficulty of sectioning for decalcified hard tissue specimens might be not due to insufficient decalcification but for the cutting skills for dens fibrous tissue composition of the hard tissue specimen. The cutting and grinding method of undecalcified preparation is an essential one for hard tissue specimens containing metals and ceramics. The adhesion method of resin embedded undecalcified specimen to slide glasses and/or acrylic plates allows easy handling the specimen during the grinding preparation not only for using a programmed machine but also for a manual preparation. The surface staining with an etching is applied for relatively thick grinding specimen. The surface staining provides as clear staining as that of in the case of thin sectioning preparation. The technical tips about the histological preparation methods should be precisely hand over by young researchers and it might not be replaced by machinery even in the feature. Furthermore, the histological methods might be an essential method for integration of knowledge about human diseases that are depended on specificity of individuals.
鹿児島大学の論文
2004-03-25

硬組織の病理組織標本作成法

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