Histopathological Study of the Effect of Periodontopathic Bacterial Lipopolysaccharide on Changes in Rat Periodontal Tissue after Penetration through the Gingival Sulcus
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概要
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Actinobacillus actinomycetemcomitans Y 4とPorphyromonas gingivalis 381のLPSがラット歯肉溝を通過して起こる歯周組織の変化を明らかにすることを目的として本研究を行った。付着上皮および結合組織に傷害を与えないように,A. actinomycetemcomitansまたはP. gingivalisのLPSの溶液(5mg/ml)をラットの第一臼歯の舌側歯肉溝に24時間おきに30分間滴下した。滴下1回,3回,7回,10回そして15回後にラットを屠殺し,病理組織学的および組織形態学的に観察し各LPSによる影響を比較した。その結果,各LPS滴下群(それぞれn=25)で炎症が観察されたが,特にA. actinomycetemcomitans LPS滴下群に強い炎症と歯周組織の変性が認められた。結合組織性付着の喪失と上皮脚の延長もA. actinomycetemcomitans LPS滴下群の方が強かった。接合上皮と上皮下結合組織の変性は1回,3回投与群に観察され,7回,10回滴下群では上皮細胞とコラーゲン線維が根表面から剥離していた。上皮脚は歯槽骨頂に向かって延長し,上皮の剥離は根尖側に広がっていた。またA. actinomycetemcomitans LPS 15回滴下群の一部の歯槽骨頂部には破骨細胞性骨吸収が観察された。これらの結果からA. actinomycetemcomitans LPSはP. gingivalis LPSよりも歯周組織破壊に対する影響が強いことが示唆された。
- 1998-06-28
著者
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Ikeda Yasuo
Department Of Internal Medicine Keio University
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KANEKO TAKASHI
Department of Environmental Health, School of Medicine, Yamanashi University
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Kaneko Takashi
Department Of Environmental Health School Of Medicine Yamanashi University
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Yoshimura A
Department Of Periodontology Nagasaki University School Of Dentistry
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HARA Yoshitaka
Department of Periodontology, Nagasaki University School of Dentistry
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KATO Ihachi
Department of Periodontology, Nagasaki University School of Dentistry
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YOSHIMURA Atsutoshi
Division of Periodontology, Nagasaki University Graduate School of Biomedical Sciences
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Ikeda Yasuo
Department Of Earth Science Hokkaido University Of Education At Kushiro
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Kato Ihachi
長崎大学 歯 2歯保存
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Kato Ihachi
Department Of Periodontology Nagasaki University School Of Dentistry
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Hara Yoshitaka
Department Of Periodontology Nagasaki University School Of Dentistry
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Iwamoto Masaaki
Department Of Geriatrics Faculty Of Medicine University Of Tokyo
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Iwamoto Masaaki
Department Of Periodontology Nagasaki University School Of Dentistry
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Yoshimura Atsutoshi
Division Of Periodontology Nagasaki University Graduate School Of Biomedical Sciences
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Yoshimura Atsutoshi
Department of Periodontology, Nagasaki University School of Dentistry
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Ikeda Yasuo
Department Of Periodontology Nagasaki University School Of Dentistry
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Kaneko T
Department Of Periodontology Nagasaki University School Of Dentistry
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Hara Yoshitaka
Department Of Lowland Farming Hokuriku National Agricultural Experiment Station
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Kaneko Takashi
Department Of Chemistry And Chemical Engineering Faculty Of Engineering Niigata University
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Kaneko Takashi
Department Of Applied Biological Science Science University Of Tokyo
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Hara Yoshitaka
Department of Periodontology,Nagasaki University School of Dentistry
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