Localization of K6-Keratin mRNA in Newborn Rat Wounds During Healing

概要

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Keratinocytes at wound sites express specific keratins, K6 and K16, which, if at all, are rarely present in healthy epidermis. Open and sutured wounds made on the backs of newborn rats (day 1) were examined in the present study, and the comparison was made of temporal and spatial K6 mRNA expression during healing of the wounds utilizing in situ hybridization method. Twelve hours subsequent to wound infliction, K6 mRNA was induced extensively in the suprabasal layer of the epidermis for either wound. With wound healing, K6 mRNA signals rapidly became concentrated at wound centers and then disappeared. K1 and K5 mRNA expression was examined by the histological sections adjacent to those for K6 mRNA. K1 mRNA signal reduction was noted at sites of strong K6 mRNA expression. K5 mRNA was expressed at constant level in the basal layer of epidermis, with no dependence on that of K1 or K6 keratin. Keratin mRNA expression and epithelialization in the present study indicated K6 mRNA to potentially serve effectively as a healing marker, because the expression coincided reasonably well with epithelialization and from late fetal stage through neonatal to adult stages of rat development. There may be no ontogenetic transition of wound healing, at least from the standpoint of the particular mode of keratin expression.
北里大学の論文
2000-12-31