Target fiberの組織化学的および電子顕微鏡的検討

概要

論文の詳細を見る
Histochemical and electron microscopic studies were performed on the target fibers which were obtained by muscle biopsy from a possible case with Charcot-Marie-Tooth disease. Three layers of abnormally stained core in the target fibers were found by several histochemical techniques such as Gomori's trichrome, NADHTR, PAS and myosin ATPase. The central zone stained poorly for oxidative enzyme and myosin ATPase. The intermediate zone showed rather intense or sometimes variable activities of these enzymes, whereas the outer zone showed little enzymatic abnormalities. Gomori's trichrome and H. E. stain revealed intense in the central zone and weak in the intermediate zone. The outer zone showed no abnormalities for these stains. Myosin ATPase stain was seemed best for detecting the target phenomenon by histochemical techniques because of stability of staining. All target fibers examined were classified as Type I fiber by using myosin ATPase stain. Diameter of traget fibers was almost normal or slightly atrophic. Electron microscopic study revealed fragmentation of the Z-band like material with lack of mitochondria and myofilaments in the central zone of the target fibers. Occasionally, an increase of mitochondria was detected in the intermediate zone. The outer zone showed almost no ultrastructural abnormalites. Double-target fiber, which was reported as a very rare occasion, was detected in this case.
北里大学の論文
1978-08-31