ラットmast cell surface antigen-1 (MASA-1)の性状と発現誘導

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Mast cells are pivotal effector cells in type I allergy, and may cross-talk to a variety of cells to participate in various phenomena such as innate immunity, chronic inflammation and tissue remodeling. However, there have been only few reports on surface proteins specifically expressed on mast cells. We have recently cloned a novel protein expressed specifically on human mast cells, the mast cell surface antigen-1 (MASA-1). MASA-1 was found to be expressed in affected tissues with subacute or chronic inflammatory diseases such as bronchiolitis obliterans organizing pneumonia and idiopathic interstitial pneumonia, but not in normal tissues. Because anti-human MASA-1 antibody had a cross-reactivity to a rat mast cell line (RBL-2H3), we investigated whether MASA-1 was expressed on mast cells in an animal model of type I allergy. MASA-1 could be detected in peritoneal mast cells of immunized rats from 3 to 25 days after antigenic challenge. Rat MASA-1, a protein with a molecular mass of about 90 kDa by immunoblotting, was found to be expressed on the surface membrane by FACS analysis. MASA-1 expression in a human mast cell line, HMC-1 cells were increased by several cytokines, like TGF-β1, TNF-α, and SCF. Consequently, it was concluded that MASA-1 is specifically expressed on activated mast cells under conditions of chronic inflammation, with a contribution of inflammatory cytokines. This study suggests that MASA-1 may be a novel marker for mast cell activation.
愛知医科大学の論文
2004-06-15

ラットmast cell surface antigen-1 (MASA-1)の性状と発現誘導

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