<原著>気管支肺胞洗浄液中の肥満細胞の動態と臨床的検討

概要

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Although the mast cells are recognized as major effector cells of type I hypersensitivity reaction, not many studies have reported on the mast cells in bronchoalveolar lavage fluid (BALF). ln this study, we investigated the population of mast cells in BALF in various pulmonary diseases by means of toluidine blue staining, human tryptase immunofluorescence method and mast cell surface antigen (MASA) immunofluorescence method. BALF was obtained from 19 patients with lung cancer, 14 sarcoidosis, 12 pneumonia, 9 pulmonary tuberculosis, 9 bronchitis, 7 non-specific interstitial pneumonia, 6 bronchial asthma, 4 bronchiolitis obliterans organizing pneumonia (BOOP), 2 non-tuberculous mycobacteriosis, 2 bronchiectasis and 9 other diseases. Number of the mast cells measured by three different methods was significantly increased in BOOP, interstitial pneumonia, bronchial asthma and sarcoidosis. Toluidin blue staining represents the granule rich mast cells, although tryptase immunofluorescence method detects all the mast cells. The difference between tryptase positive cells and toluidine blue positive cells means the immature mast cells or degranulated mast cells. This difference was prominent in BOOP, interstitial pneumonia, and sarcoidosis, and was correlated with MASA positive cells. 0ur results suggested that MASA should be a marker for the activated mast cells.
愛知医科大学の論文
2003-03-15