遺伝子組換えタマネギに生分解性プラスチック原料を生合成させる試みII(2003-2004年度神奈川大学共同研究奨励助成論文)

概要

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We have been conducting experiments for the purpose of exploiting new techniques to produce beneficial materials, such as bkrdegradable plastic, within genetically modified (GM) onion cells. A transforming procedure to produce GM onions was established, in which Agrobacterium tumefacience was used to introduce foreign genes into the onion genome. As a result, we obtained GM onion seedlings, expressing introduced Green Fluorescent Protein (GFP) gene, by selecting on hygromycine-containing medium. An Arabidopsis gene, AtMTP1 encoding vacuole membrane protein, was examined for its capability as a guide to transfer foreign gene products into vacuoles. AtMTP1-GFP fusion genes were delivered into the epidermis cells of an onion bulb by a particle gun. Under a fluorescence microscope, the gene product was detected on vacuole membranes. Our present results, together with the former results, indicated that it is possible to express foreign genes under control of an alliinase gene promoter, and accumulate the gene product in the vacuole at a high level.
神奈川大学の論文
2005-05-25