ポリエステルの微生物分解 : Ralstonia metalliduransにおけるPHB結合蛋白質の役割の検討(2005年度神奈川大学総合理学研究所協同研究助成論文)

概要

論文の詳細を見る
The genes of two poly(3-hydroxybutyrate) inclusion body-binding proteins (GAP1 and GAP2) were cloned from Raltsonia metallidurans CH34, and their gene products were purified. The molecular masses of GAP1 and GAP2 were 24 kDa and 18 kDa, respectively. GAP1 comprised the major part of proteins in the PHB inclusion body of R. metallidurans and its amino acid sequence was quite similar to that of Phasin of R. eutropha H16. These results indicate that GAP1 probably play the role of the major protective protein of the inclusion body. The content of GAP2 in the PHB inclusion body of R. metallidurans was very small. Although GAP2 accelerated the degradation of the PHB inclusion body in a similar manner to Apd from Rhodospirillum rubrum, the physiological role of GAP2 was not clear.
2006-05-25