Fluorescent in situ hybridization(FISH)法によるシロイヌナズナの花粉母細胞での減数第一分裂期染色体の動態解析(2003-2004年度神奈川大学共同研究奨励助成論文)

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Since insertion mutagenesis methods, which enabled us to identify the mutagenized genes routinely, were developed for plants, Arabidopsis thaliana has been playing a central role in plant meiosis research. Though several techniques to analyze meiotic chromosome behavior have been introduced into Arabidopsis research since Ross et al. reported the method to observe male meiotic chromosomes of this plant through light microscope in 1996 (Chromosome Res. 4-507-516), intimate analysis of the chromosome behavior has not been accomplished. Taking advantage of the recent development of new nucleotides labeled with fluorescent dyes, we investigated chromosome behavior during male meiosis by multicolor FISH. Telomeres found around nucleoli in premeiotic interphase cells dispersed after entering meiosis, then clustered in a bouquet-like configuration. Statistically, telomeres of homologous chromosomes paired earlier than centromeres, but when respective chromosomes were examined, the telomeres were not always quick to pair. At early prophase I, possibly at around the zygotene stage, the signals from telomeres reduced to less than ten. This reduction suggests that the paired telomeres of homologous chromosomes temporally associate with other telomeres to look for their real partners. When homologous chromosomes separated at anaphase I, telomeres were always last to segregate. This suggested that there was unknown interaction between the telomeres of homologs, connecting them until anaphase I started.
神奈川大学の論文
2005-05-25

Fluorescent in situ hybridization(FISH)法によるシロイヌナズナの花粉母細胞での減数第一分裂期染色体の動態解析(2003-2004年度神奈川大学共同研究奨励助成論文)

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