薬剤処理やアンチセンス核酸注射によるゼブラフィッシュの後天的アルビノ個体の作製方法の探索(2007年度神奈川大学総合理学研究所共同研究助成論文)

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■原著■ 2007 年度神奈川大学総合理学研究所共同研究助成論文Zebrafish is a good model organism for developmental biology and biomedicalresearch. In its early developmental stages, zebrafish is very transparent, and the speed ofdevelopment is rapid, however, its larva is highly pigmented, and thus it is almostimpossible to observe the internal organs using non-invasive methods. Here we report theeffects of antisense morpholino oligonucleotides (MO) and organic compounds which targetzebrafish melanogenesis. Antisense MO for zebrafish tyrosinase and phenyl-thiourea(PTU) are highly effective to reduce the pigmentation of melanosomes in melanophores.Arbutin and Kojic acid are partially effective, and Fullerene C60 is not effective forinhibiting zebrafish melanogenesis. Except for pigmentation, all these reagents did notdisrupt normal embryogenesis and the left-right specification of the heart. After 8-daytreatment with PTU, we succeeded in visualizing the shape of the brain ventricle byinjecting with Qdot 655 (red nanocrystal). The inhibition of melanogenesis is useful for thestudy of brain development using zebrafish larvae.
神奈川大学の論文