Bovine Myoblast Differentiation

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概要

• 論文の詳細を見る

• Satellite cells are involved in postnatal myogenesis and in muscle hypertrophy. A better understanding of the mechanisms of myogenesis is useful to improve the muscle production in farm animals. Herein, we show the cytokine effects on the myogenesis in bovine myoblast cultures. Acidic fibroblast growth factors (aFGF) and interleukin 1 (IL-1) stimulated the cell proliferation, and insulin-1ike growth factor-I (IGF-I) stimulated to form multinucleated myotubes. Thus, it was possible to regulate the bovine myoblast differentiation by aFGF, IL-1 and/or IGF-I. Using the culture system, the expression of myosin heavy chain (MyHC) isoforms was detailed in bovine myoblasts during the differentiation. It was immunohistochemically confirmed that bovine myoblasts expressed β/slow-type MyHC (MyHC-slow), fast-type MyHC (MyHC-fast) and developmental-type MyHC (MyHC-dev) isoforms. Furthermore, the expression of fast 2a and β/slow MyHC mRNA was recognized in the cultures of bovine myoblasts. The results support the existence of bovine myoblast phenotypes that express differentially MyHC isoforms.

• 東北大学の論文
• 2004-11-25

著者

• Yamaguchi Takahiro

  Department Of Animal Biology Graduate School Of Agricultural Science Tohoku University

• Yamaguchi T

  Graduate School Of Agricultural Science Tohoku University

• Yamaguchi T

  Shizuoka Univ. Hamamatsu

• Yamaguchi Takahiro

  Laboratory of Functional Morphology, Department of Animal Production Science, Graduate School of Agr

• WATANABE Kouichi

  Department of Animal Biology, Graduate School of Agricultural Science, Tohoku University

• OHWADA Shyuichi

  Department of Animal Biology, Graduate School of Agricultural Science, Tohoku University

• ASO Hisashi

  Department of Animal Biology, Graduate School of Agricultural Science, Tohoku University

• WATANABE Kouichi

  Laboratory of Functional Morphology, Department of Animal Science, Graduate School of Agricultural S

• OHWADA Shyuichi

  Laboratory of Functional Morphology, Department of Animal Science, Graduate School of Agricultural S

• ASO Hisashi

  Laboratory of Functional Morphology, Department of Animal Science, Graduate School of Agricultural S

• HAYASHI Sin-ichiro

  Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University

• NARA Hidetoshi

  Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University

• Hayashi Sin-ichiro

  Laboratory Of Functional Morphology Department Of Animal Science Graduate School Of Agricultural Sci

• Watanabe Kouichi

  Department Of Animal Biology Graduate School Of Agricultural Science Tohoku University

• Aso Hisashi

  Department Of Animal Biology Graduate School Of Agricultural Science Tohoku University

• Aso H

  National Inst. Animal Ind. Ibaraki Jpn

• Aso Hisashi

  Laboratory Of Cellular Biology Laboratory Department Of Animal Physiology National Institute Of Anim

• Nara Hidetoshi

  Laboratory Of Functional Morphology Graduate School Of Agricultural Science Tohoku University

• Ohwada Shyuichi

  Tohoku Univ. Sendai Jpn

• Ohwada S

  Department Of Animal Biology Graduate School Of Agricultural Science Tohoku University

• Yamaguchi T

  Department Of Animal Biology Graduate School Of Agricultural Science Tohoku University

• Ohwada Shyuichi

  Laboratories Of Animal Breeding And Animal Morphology

• Watanabe K

  Tohoku Univ. Sendai‐shi Jpn

• Yamaguchi Takahiro

  Laboratory Of Functional Morphology Department Of Animal Production Science Faculty Of Agriculture T

• YAMAGUCHI Takahiro

  Laboratory of Animal Monphology, Faculty of Agriculture, Tohoku University

• NARA Hidetoshi

  Laboratory of Animal Genetics, Gifu University

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