実験動物用マイクロCTを用いたrhBMP-2・アテロコラーゲンゲルによるウサギの顎骨欠損部再建の経時的観察

概要

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A rabbit experimental mandibular defect was reconstructed with rhBMP-2 and atelocollagen gel, and examined using μCT (R_mCT, Rigaku mechatronics, Tokyo) in vivo and histological techniques. Using eight rabbits, we made an experimental mandibular defect and filled it with 1% atelocollagen gel including rhBMP-2 10μg (Astellas Pharma Inc.). In μCT observation, the density was slightly elevated at the bone marrow side at day 5, and the phenomenon gradually expanded during the course of this experiment. Histologically, mesenchymal cell proliferation and immature bone formation occurred at one week, and mature bone gradually increased and filled in at four weeks. The histological data suggests that atelocollagen gel is effective as carrier of rhBMP-2. The μCT observation is extremely useful for follow-up of such reconstruction of animal bone defect model.
硬組織再生生物学会の論文