BMPによる異所性骨組織の誘導過程におけるTGF-βの発現

概要

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Based upon only general histological study, many researchers have indicated that heterotopic bone tissue induced by bone morphogenetic protein (BMP) was formed by endochondral ossification. However, it is not so clear whether the osteogenesis is normal. Therefore, we investigated not only the process of heterotopic bone tissue formation induced by BMP histopathologically, but also the manifestation of transforming growth factor beta 1 (TGF-β1) immunohistochemically. Gelatine capsule containing 5 mg BMP (partially punfied) was implanted into the subfascia of the femur of ddY mice under general anesthesia. The implants were removed from the mice at 1 to 4 weeks after the operation, and studied histopathologically and immunohistochemically using anti-human TGF-β1 antibody. TGF-β1 was detected in various cells proliferating during the endochondral osteogenesis course: In 1 week specimen TGF-β1 was detected in chondrocytes and matrices, and in two weeks it appeared in osteoblasts and the matrices. In three weeks, the peptide was shown in osteoblasts and osteosytes, and newly formed bone marrow tissues. Positive reactions remained only in a part of the matrices in four weeks specimen. There was, however, a slight difference in the manifestation of TGF-β1 in heterotopic osteogenesis between BMP and the physiological response.
硬組織再生生物学会の論文