Effects of Novel Ethacrynic Acid Derivatives on Human Trabecular Meshwork Cell Shape, Actin Cytoskeletal Organization, and Transcellular Fluid Flow(Pharmacology)
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概要
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To determine efficacy and therapeutic index in the context of ocular hypotensive activity of the new ethacrynic acid (ECA) derivatives of the 8000 series (SA8248 and SA8389), 9000 series (SA9000, SA9622 and SA9995) and ticrynafen, we undertook a comparative evaluation of the dose-dependent effects of these compounds on human trabecular meshwork (HTM) cell shape, actin cytoskeletal organization, focal adhesions and transcellular fluid flow. Responses were either scored using an arbitrary scale of 1-5 or quantified. Compounds of the 9000 series (SA9995>SA9000>SA9622) were found to be 14- to 20-fold more potent than ECA, ticrynafen or analogs from the 8000 series (SA8389>SA8248) in terms of ability to induce cell shape alterations in HTM cells. Similarly, compounds of the 9000 series (SA9995>SA9622>SA9000) were found to be much stronger (2 to 20 fold) than ECA, ticrynafen or analogs of the 8000 series in terms of affecting decreases in actin stress fiber content in HTM cells. Analogs of the 9000 series (SA9622>SA9995>SA9000) were also observed to be 8 to 10 fold more potent than ECA (SA8389>ECA>SA8248>ticrynafen) at eliciting decreases in cellular focal adhesions. Interestingly, analogs of the 9000 series (SA9000>SA9622>SA9995) and SA8248 demonstrated a huge increase (by many folds) in transcellular fluid flow of HTM cell monolayers as compared to ECA and ticrynafen. Collectively, these analyses revealed that the structural modification of ECA improves its ocular hypotensive efficacy, indicating that the SA9000 series compounds might be promising novel ocular hypotensive drugs.
- 公益社団法人日本薬学会の論文
- 2005-12-01
著者
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Epstein David
Department Of Ophthalmology Duke University Medical Center & Health System
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Epstein David
参天製薬奈良研究開発センター
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Ichikawa M
Healthcare Research Institute Wakunaga Pharmaceutical Co. Ltd.
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SHIMAZAKI Atsushi
Research and Development Center, Santen Pharmaceutical Co., Ltd.
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RAO Ponugoti
Department of Ophthalmology, Duke University Medical Center & Health System
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ICHIKAWA Masaki
Research and Development Center, Santen Pharmaceutical Co., Ltd.
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ALVARADO Jorge
Department of Ophthalmology, University of California
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Rao Ponugoti
Department Of Ophthalmology Duke University Medical Center & Health System
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Alvarado Jorge
Department Of Ophthalmology University Of California
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Shimazaki Atsushi
Research And Development Center Santen Pharmaceutical Co. Ltd.
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Ichikawa Masaki
Research And Development Center Santen Pharmaceutical Co. Ltd.
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