抵抗性ニンジン培養細胞によるベンスルフロンメチルの吸収と解毒代謝

概要

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Carrot (Daucus carota L. cv. US-Harumakigosun) cell suspension cultures adapted to grow in 10^<-8> M of bensulfuron methyl (BSM) were cultured in LS-medium by subculturing every 7 days. The absorption and the metabolic change of ^<14>C-BSM in resistant and normal cells were investigated. ^<14>C-BSM at 10-6 or 10-'M were added to the cells 4 days after subculture at their linear growth phase. ^<14>C-BSM was rapidly absorbed during the initial 30-60 min. The absorption rates of BSM by normal and resistant cells were not significantly different, though they were maximally 22 and 18% of the initially added amount at I hr, respectively. For metabolism study, ^<14>C-BSM and its metabolites were extracted by 80% acetone. Aqueous fraction after evaporation was partitioned by dichloromethane at pH 7 and then 3. ^<14>C compounds of each fraction were analyzed with TLC. ^<14>C-BSM was partially metabolized to O-demethyl BSM, sulfonamide, homosaccharin, water soluble and residual compounds in both cell groups to nearly the same extent. These results suggested that the absorption and metabolism of BSM did not much differ between the normal and resistant carrot suspension cultured cells.
日本雑草学会の論文
1993-05-28