ヒメイヌビエ発芽初期のATP測定における種子消毒について

概要

論文の詳細を見る
1 . Three ATP extraction methods for Echinochloa crus-galli var. praticola imbibed seeds were tested : 1) boiling intact seeds 2) boiling cracked seeds and 3) grinding seeds with or without 5% TCA. With the two boiling methods a very small amount of ATP was detected. The grinding method accomplished in a very short time using Triton X-100 as an extract solution for ATP gave the best results. 2 . Three methods of cleaning for various durations were compared in E. crus-galli var. praticola seeds : 1) dipping in 70% ethyl alcohol (EtOH), 2) dipping in 1% NaClO solution and 3) exposure to formaldehyde gas. The results showed that dipping in 70% EtOH or exposing to formaldehyde gas for 3 minutes is an appropriate means of cleaning E. crus-galli var. praticola seed for ATP assay.
日本雑草学会の論文
1989-12-25