Expression Characteistics of CS-ACS1, CS-ACS2 and CS-ACS3, Three Members of the 1-Aminocyclopropane-1-Carboxylate Synthase Gene Family in Cucumber (Cucumis sativus L.)Fruit under Carbon Dioxide Stress

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概要

• 論文の詳細を見る

• We investigated the expression pattern of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes, CS-ACS1, CS-ACS2 and CS-ACS3 in cucumber (Cucumis sativus L.) fruit under CO_2 stress. CO_2 stress-induced ethylene production paralleled the accumulation of only CS-ACS1 transcripts which disappeared upon withdrawal of CO_2. Cycloheximide inhibited the CO_2 stress-induced ethylene production but superinduced the accumulation of CS-ACS1 transcript. At higher concentrations, cycloheximide also induced the accumulation of CS-ACS2 and CS-ACS3 transcripts. In the presence of CO_2 and cycloheximide, the accumulation of CS-ACS2 transcript occurred within 1 h, disappeared after 3 h and increased greatly upon withdrawal of CO_2. Inhibitors of protein kinase and types 1 and 2A protein phosphatases which inhibited and stimulated, respectively, CO_2 stress-induced ethylene production had little effect on the expression of these genes. The results presented here identify CS-ACS1 as the main ACC synthase gene responsible for the increased ethylene biosynthesis in cucumber fruit under CO_2 stress and suggest that this gene is a primary response gene and its expression is under negative control since it is expressed by treatment with cycloheximide. The results further suggest that the regulation of CO_2 stress-induced ethylene biosynthesis by reversible protein phosphorylation does not result from enhanced ACC synthase transcription.

• 日本植物生理学会の論文

著者

• Mwaniki Mercy

  Graduate School Of Natural Science And Technology Okayama University:department Of Food Science And

• Kubo Y

  Graduate School Of Natural Science And Technology Okayama University

• Mathooko F

  Department Of Food Science And Technology Faculty Of Agriculture Jomo Kenyatta University Of Agricul

• Kubo Yasutaka

  Faculty Of Agriculture Okayama University

• Mathooko Francis

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Mwaniki Mercy

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Nakatsuka Akira

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Shiomi Shinjiro

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Kubo Yasutaka

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Inaba Akitsugu

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Nakamura Reinosuke

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Inaba Akitsugu

  Graduate School Of Natural Science And Technology Okayama University

• Inaba Akitsugu

  Laboratory Of Postharvest Agriculture Faculty Of Agriculture Okayama University

• Nakatsuka Akira

  Laboratory Of Postharvest Agriculture Faculty Of Agriculture Okayama University

• Shiomi Shinjiro

  Faculty Of Food Culture Kurashiki Sakuyo University

• Nakano Ryohei

  Okayama Univ. Okayama Jpn

関連論文

• Monoclonal Antibodies Spicific for NADH-Ferricyanide Reductase Domain of Spinach Leaf Nitrate Reductase
• Microsatellite and CAPS Markers for Ethylene-related Genes, 1-Aminocyclopropane-1-Carboxylic Acid(ACC)Synthase and ACC Oxidase Genes, and their Variation in Melon(Cucumis melo L.)
• β-Galactosidase and α-L-Arabinofuranosidase Activities and Gene Expression in European and Chinese Pear Fruit During Ripening
• Expression Characteistics of CS-ACS1, CS-ACS2 and CS-ACS3, Three Members of the 1-Aminocyclopropane-1-Carboxylate Synthase Gene Family in Cucumber (Cucumis sativus L.)Fruit under Carbon Dioxide Stress
• Expression and Internal Feedback Regulation of ACC Synthase and ACC Oxidase Genes in Ripening Tomato Fruit
• Studies on the Internal Feedback Regulation of Ethylene Biosynthesis and Signal Transduction during Fruit Ripening, and the Improvement of Fruit Quality
• Characterization of Carbon Dioxide Stress-Induced Ethylene Biosynthesis in Cucumber (Cucumis sativus L.) Fruit

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