Microsatellite and CAPS Markers for Ethylene-related Genes, 1-Aminocyclopropane-1-Carboxylic Acid(ACC)Synthase and ACC Oxidase Genes, and their Variation in Melon(Cucumis melo L.)
スポンサーリンク
概要
- 論文の詳細を見る
To establish DNA markers for ethylene-related genes in melon(Cucumis melo L.), we searched for microsatellite and cleaved amplified polymorphic sequence(CAPS)polymorphisms in two ACC synthase genes(CMe-ACS1, CMe-ACS2)and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), and found two CAPSs and six microsatellite markers. Five markers were detected in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeats(TA)was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of(T)_n and(A)_n were also detected in CMe-ACS2. Specific CAPS and microsatellites for vars.makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.
- 日本育種学会の論文
著者
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Akashi Yukari
Faculty Of Agriculture Okayama University
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Akashi Y
Faculty Of Agriculture Okayama University
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Kato Kenji
Faculty of Agriculture, Okayama University
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Shiomi Shinjiro
Faculty of Food Culture, Kurashiki Sakuyo University
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Kubo Yasutaka
Faculty of Agriculture, Okayama University
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Masuda Masaharu
Faculty of Agriculture, Okayama University
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Kubo Y
Graduate School Of Natural Science And Technology Okayama University
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Kubo Yasutaka
Faculty Of Agriculture Okayama University
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Masuda M
Faculty Of Agriculture Okayama University
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Masuda Masaharu
Faculty Of Agriculture Okayama University
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Shiomi Shinjiro
Faculty Of Food Culture Kurashiki Sakuyo University
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Kato Kenji
Faculty Of Agriculture Okayama University
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