Expression and Internal Feedback Regulation of ACC Synthase and ACC Oxidase Genes in Ripening Tomato Fruit

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概要

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• We have examined whether or not a positive feedback regulation of gene expression for 1-aminocyclopropane-l-carboxylate (ACC) synthase and ACC oxidase also operates in ripening tomato (Lycopersicon esculentum) fruit during the burst of ethylene production. Two cDNA fragments for ACC synthase and one for ACC oxidase were cloned with high homology to already known genes involved in ethylene biosynthesis in ripening tomato fruit. Accumulation of mRNAS Which hybridize to these cDNA probes were induced in mature green fruit within two days by treatment with propylene. In the fruit ripened from the turning stage, red color development, ethylene production, ACC content, and activities of ACC synthase and ACC oxidase increased as maturity progressed. The abundance of two ACC synthase and one ACC oxidase mRNAS in the fruit increased from the turning to pink stage and were followed by a slight decline towards the red stage. These in-creases in mRNAS abundance with ripening were prevented to a large extent by treatment with the ethylene action inhibitor, 1-methylcyclopropene (MCP). This was most pronounced in the fruit treated with MCP at the turning stage, in which the accumulation of ACC synthase and ACC oxidase transcripts was almost completely eliminated in the first two d, precisely the same stage at which the control fruit had the greatest level of each mRNA accumulation. The inhibition of transcript accumulation recovered to the control level within two to four d. MCP also decreased ethylene biosynthetic activity, although this decrease did not reflect the reduction in the mRNAS accumulation. These results suggest that a strong positive feedback regulation is involved in ethylene biosynthesis at the gene transcriptional level in tomato fruit, even at the stage with a burst of ethylene production. Key words: ACC oxidase (EC I .4.3) - ACC synthase

• 日本植物生理学会の論文

著者

• Kubo Y

  Graduate School Of Natural Science And Technology Okayama University

• Kubo Yasutaka

  Faculty Of Agriculture Okayama University

• Nakatsuka Akira

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Shiomi Shinjiro

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Kubo Yasutaka

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Inaba Akitsugu

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Inaba Akitsugu

  Graduate School Of Natural Science And Technology Okayama University

• Inaba Akitsugu

  Laboratory Of Postharvest Agriculture Faculty Of Agriculture Okayama University

• Nakatsuka Akira

  Laboratory Of Postharvest Agriculture Faculty Of Agriculture Okayama University

• Shiomi Shinjiro

  Faculty Of Food Culture Kurashiki Sakuyo University

関連論文

• Monoclonal Antibodies Spicific for NADH-Ferricyanide Reductase Domain of Spinach Leaf Nitrate Reductase
• Microsatellite and CAPS Markers for Ethylene-related Genes, 1-Aminocyclopropane-1-Carboxylic Acid(ACC)Synthase and ACC Oxidase Genes, and their Variation in Melon(Cucumis melo L.)
• β-Galactosidase and α-L-Arabinofuranosidase Activities and Gene Expression in European and Chinese Pear Fruit During Ripening
• Expression Characteistics of CS-ACS1, CS-ACS2 and CS-ACS3, Three Members of the 1-Aminocyclopropane-1-Carboxylate Synthase Gene Family in Cucumber (Cucumis sativus L.)Fruit under Carbon Dioxide Stress
• Expression and Internal Feedback Regulation of ACC Synthase and ACC Oxidase Genes in Ripening Tomato Fruit
• Studies on the Internal Feedback Regulation of Ethylene Biosynthesis and Signal Transduction during Fruit Ripening, and the Improvement of Fruit Quality
• Characterization of Carbon Dioxide Stress-Induced Ethylene Biosynthesis in Cucumber (Cucumis sativus L.) Fruit

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