Characterization of Carbon Dioxide Stress-Induced Ethylene Biosynthesis in Cucumber (Cucumis sativus L.) Fruit

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概要

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• In the present study, we have investigated the mechanism through which carbon dioxide induce ethylene biosynthesis in cucumber (Cucumis sativus L.) fruit. A series of inhibitors were tested in order to determine the involvement of de novo protein synthesis, new mRNA synthesis, protein kinase activity, and phosphoprotein phosphatase activity in carbon dioxide stress-induced ethylene biosynthesis. Carbon dioxide stimulated ethylene production through the induction of both 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase activities. Induction of ACC synthase activity led to accumulation of ACC. Cycloheximide blocked carbon dioxide stress-induced ethylene biosynthesis while cordycepin had no effect. Dibucaine and 6-dimethylaminopurine, inhibitors of protein kinases, blocked carbon dioxide stress-induced increases in ethylene production, ACC synthase activity, and ACC content but not the increase in ACC oxidase activity. Cantharidin, a potent inhibitor of protein phosphatase type 1 and type 2A, stimulated carbon dioxide stress-induced ethylene production, ACC synthase activity, and ACC accumulation but had no additional effect on carbon dioxide stress-induced ACC oxidase activity. In the presence of dibucaine or cycloheximide, the stimulation of carbon dioxide stress-induced ethylene biosynthesis by cantharidin was blocked. These data suggest that carbon dioxide stress-induced ethylene biosynthesis may be regulated posttranscriptionally and that the induction involves the synthesis of novel protein(s) in the cytosol. The results further identify protein phosphorylation and dephosphorylation as requirements in one or more of the steps involved in the carbon dioxide signal transduction pathway that leads to induction of ethylene biosynthesis and, in particular to the induction of ACC synthase, presumably the key enzyme in the ethylene biosynthetic pathway.

• 日本植物生理学会の論文

著者

• Mathooko F

  Department Of Food Science And Technology Faculty Of Agriculture Jomo Kenyatta University Of Agricul

• Mathooko Francis

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Nakamura Reinosuke

  Laboratory of Postharvest Agriculture, Faculty of Agriculture, Okayama University

• Inaba Akitsugu

  Graduate School Of Natural Science And Technology Okayama University

• Inaba Akitsugu

  Laboratory Of Postharvest Agriculture Faculty Of Agriculture Okayama University

• Nakano Ryohei

  Okayama Univ. Okayama Jpn

関連論文

• β-Galactosidase and α-L-Arabinofuranosidase Activities and Gene Expression in European and Chinese Pear Fruit During Ripening
• Expression Characteistics of CS-ACS1, CS-ACS2 and CS-ACS3, Three Members of the 1-Aminocyclopropane-1-Carboxylate Synthase Gene Family in Cucumber (Cucumis sativus L.)Fruit under Carbon Dioxide Stress
• Expression and Internal Feedback Regulation of ACC Synthase and ACC Oxidase Genes in Ripening Tomato Fruit
• Studies on the Internal Feedback Regulation of Ethylene Biosynthesis and Signal Transduction during Fruit Ripening, and the Improvement of Fruit Quality
• Characterization of Carbon Dioxide Stress-Induced Ethylene Biosynthesis in Cucumber (Cucumis sativus L.) Fruit

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