Formation of singlet molecular oxygen in illuminated chloroplasts. Effects on photoinactivation and lipid peroxidation
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概要
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The formation of singlet molecular oxygen (^1O_2) in illuminated chloroplasts and the effects of ^1O_2 on oxidation or destruction of components and functional integrity of chloroplasts were studied. The rate of photoreduction of 2,6-dichloroindophenol (DCIP) and the extent of the 515-nm absorbance change were decreased by light irradiation and by xanthine oxidase treatment. Malondialdehyde (MDA) formation, an indicator of lipid peroxidation, was observed in the light-irradiated chloroplast fragments, but not in the xanthine-xanthine oxidase-treated chloroplast fragments. MDA formation was absent under anaerobic conditions. MDA formation was stimulated when electron transfer on the oxidizing side of photosystem II (or I) was inhibited or inactivated by carbonylcyanide m-chlorophenyl-hydrazone (CCCP), Tris-treatment, prolonged illumination, etc. MDA formation was also stimulated by 3-(3,4-dichlorophenyl)-1, 1-dimethylurea (DCMU) when electron transfer between water and the reaction center of photosystem II was intact. CCCP-or DCMU-stimulated MDA formation was inhibited by 1,4-diazabicyclo[2.2.2]octane, a quencher of singlet molecular oxygen (^1O_2). DCMU and electron donors for photo-system II, such as ascorbate, hydroquinone and semicarbazide, inhibited MDA formation by illuminantion of the Tris-washed or CCCP-poisoned chloroplast fragments. Reduced DCIP, an electron donor for photosystem I, also inhibited MDA formation in the presence of DCMU. These results lead to the conclusion that MDA formation was initiated by ^1O_2 formed in illuminated chloroplasts. Of the three mechanisms discussed for ^1O_2 generation in illuminated chloroplasts, the formation by the electron transfer reaction between superoxide anion radical and the oxidant formed on the oxidizing side of photosystem II (or I) is most important.
- 日本植物生理学会の論文
著者
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Nishimura Mitsuo
Departmcnt Of Biology Faculty Of Science Kyushu University
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Takahama Umeo
Departmcnt Of Biology Faculty Of Science Kyushu University
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Takahama Umeo
Department Of Biology Faculty Of Science Kyushu University
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