Effects of ethanol on the interaction of photosynthetic processes in spinach chloroplasts
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概要
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The interaction and coupling between photosynthetic processes were studied in ethanol-treated chloroplasts (in the absence of ethanol) or in the presence of ethanol. Light-induced H+ uptake and photophosphorylation were suppressed and electron transfer was enhanced by ethanol treatment or in the presence of ethanol (10-25%). Dark recoveries of the H^+ uptake and the 515-nm absorbance change were accelerated by ethanol. The half-effective concentrations of ethanol for these processes were higher in the ethanol-treated chloroplasts than when ethanol was present in the reaction mixture. The maximum rate of electron transfer in the ethanol-treated chloroplasts, which was at the same level as that of the control with an uncoupler, was not affected by uncouplers. The marked acceleration of recovery of the 515-nm absorbance change by SCN- or valinomycin plus K^+ in the untreated chloroplasts was much smaller in the ethanol-treated chloroplasts or in the presence of ethanol. The ethanol-treated preparation had the same characteristics as those of the control, in chlorophyll fluorescence, light-intensity dependence of electron transfer (compared with the control with an uncoupler), and sensitivity to sucrose osmolarity except for a slight increase of the packed volume. Under the present conditions, the ethanol treatment mainly induced an increase of permeability of thylakoid membranes to ions. In chloroplasts treated with ethanol at various concentrations, the relationships of the uncoupler-stimulated part of electron transfer with the dark half-recovery time of H^+ uptake and with photophosphorylation were linear. Logarithms of the photophosphorylation and the extent of H^+ uptake had a linear relationship with a slope of about 3. This slope may indicate the stoichiometry of H^+/ATP.
- 日本植物生理学会の論文
著者
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Nishimura Mitsuo
Departmcnt Of Biology Faculty Of Science Kyushu University
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Masamoto Kazumori
Department Of Biology Faculty Of Science Kyushu University
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