Inhibitory Effects of Active Compounds from Punica granatum Pericarp on Verocytotoxin Production by Enterohemorrhagic Escherichia coli O157:H7
スポンサーリンク
概要
- 論文の詳細を見る
Punica granatum (P. granatum) Linn. pericarp has been commonly employed as a crude drug in Thai traditional medicines for the treatment of diarrhea. The antibacterial activity of extracts from P. granatum pericarp against different strains of enterohemorrhagic Escherichia coli (E. coli) O157:H7 and other strains of enterohemorrhagic E. coli were investigated. Successive chloroform, 95% ethanol, and water extracts of the plant were examined. The ethanolic extract was found to be the most effective against all the strains examined. Both the ethyl acetate and n-butanol fractions of P. granatum pericarp were demonstrated to have high activity with the highest minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of 0.05 and 0.05 and 0.39 and 0.19mg/ml, respectively. The inhibitory effects of both fractions on the production of verocytotoxin (VT)1 and VT2 by enterohemorrhagic E. coli O157:H7 was observed at very low concentrations of 1/10 of the MIC value (0.05-0.09mg/ml, respectively). Phytochemical screening of the ethanolic extracts demonstrated that they contain flavonoids, sterols, triterpenes, phenols, and tannins. Our findings suggest that an appropriate bioactive compound may be developed from P. granatum pericarp as alternative treatment for this E. coli O157:H7 infection. The crude plant may also be administered to prevent VT production in the human intestine to solve the problem of subinhibitory effects from the use of antibiotics.
- 公益社団法人日本薬学会の論文
- 2005-10-01
著者
-
Iida Tetsuya
大阪市立大学 工学研究科応用化学
-
Honda Takeshi
大阪市立大学 工学研究科応用化学
-
Honda T
Department Of Bacterial Infections Research Institute For Microbial Disease Osaka University
-
Honda T
Department Of Bacterial Infection Research Institute For Microbial Diseases Osaka University
-
Iida Tetsuya
Research Institute Of Electronics Shizuoka University
-
Honda Takeshi
Department Of Bacterial Infections Research Institute For Microbial Disease Osaka University
-
Limsuwan Surasak
Dep. Of Microbiology Fac. Of Sci. Prince Of Songkla Univ.
-
Limsuwan Surasak
Department Of Microbiology Faculty Of Science Prince Of Songkla University
-
Voravuthikunchai Supayang
Natural Products Res. Center And Dep. Of Microbiology Fac. Of Sci. Prince Of Songkla Univ.
-
Honda Takeshi
Research Institute For Microbial Diseases Osaka University
-
Sririrak Treechada
Department of Microbiology, Faculty of Science
-
Supawita Thanomjit
Department of Pharmacognosy, Faculty of Pharmacy, Prince of Songkla University
-
Honda Takeshi
Department Of Bacterial Infections Research Institute For Microbial Diseases Osaka University
-
Sririrak Treechada
Department Of Microbiology Faculty Of Science
-
Supawita Thanomjit
Department Of Pharmacognosy Faculty Of Pharmacy Prince Of Songkla University
-
Iida Tetsuya
Research Institute for Microbial Diseases, Osaka University
関連論文
- 海外旅行者から分離された新しい血清型組み合わせを有する腸炎ビブリオ
- DNAマイクロアレイを用いたO3:K6血清型腸炎ビブリオの比較ゲノム解析
- 腸炎ビブリオ Pathogenicity Island の領域決定
- 腸炎ビブリオ耐熱性溶血毒変異体の解析 : 膜脂質に対する結合性と多量体形成能
- 腸炎ビブリオのTTSS2遺伝子群の発現制御機構の解析
- 腸炎ビブリオのTTSS1遺伝子群の発現制御機構の解析
- 腸炎ビブリオのキチン粒子への定着における type IV pili の機能
- Noise reduction effects of column-parallel multiple sampling circuits (情報センシング)
- 大学等におけるバイオセイフティーの確保について
- ネットで検索できる病原細菌データベース
- 腸管細菌感染症の病態生理 (今月の主題 食中毒)
- 腸管出血性大腸菌感染症の現況 (あゆみ 変貌する細菌感染症)
- 腸管病原性細菌に対する Enterococcus faecium BIO の増殖抑制効果の検討
- 腸管出血性大腸菌(いわゆるO157)と牛肉の安全性
- 1. luxオペロンの水平転移(第407回研究協議会研究発表要旨,ビタミンB研究委員会)
- Aeromonas hydrophila targets big endothelin-3 in a thermo-regulated manner
- Providencia alcalifaciens の3型分泌装置依存的細胞毒性機構の解析
- 腸管出血性大腸菌が産生するエフェクターNleAの宿主細胞内修飾
- 微生物の管理
- 新しい食中毒菌としてのプロビデンシア・アルカリファシエンス
- 感染症との新たな闘い(11)腸管感染症の現況とその治療
- OP-123 グラム陰性菌より分泌されるAexUタンパク質を用いたヒト前立腺癌細胞株PC3における抗腫瘍効果の検討(前立腺腫瘍/基礎1,一般演題口演,第97回日本泌尿器科学会総会)
- 腸炎ビブリオのOK血清型組み合わせの現況
- In Planta Production of Immunogenic Poliovirus Peptide Using Tobacco Mosaic Virus-Based Vector System(PLANT BIOTECHNOLOGY)
- N-Linked Glycan Structures of Human Lactoferrin Produced by Transgenic Rice
- Human N-Acetylglucosaminyltransferase I. : Expression in Escherichia coli as a Soluble Enzyme, and Application as an Immobilized Enzyme for the Chemoenzymatic Synthesis of N-Linked Oligosaccharides
- エルシニア
- セレウス菌
- III.感染症の類型-疾病概念及び対応- 3.3類感染症(腸管出血性大腸菌感染症)
- 腸管出血性大腸菌O157 (特集 食中毒を考える)
- 3P011 腸炎ビブリオの産出する耐熱性溶血毒TDHの構造(蛋白質-構造,第48回日本生物物理学会年会)
- Localization Profiles of Circadian Neuropeptide PDF in Brain of the Cricket Gryllus Bimaculatus
- 15.luxオペロン所在場所(座)の比較ゲノミクス(第395回ビタミンB研究委員会研究発表要旨)
- Production and Partial Characterization of Antibody to Cord Factor (Trehalose 6, 6'-Dimycolate) in Mice
- クリーニングによるセレウス菌除菌効果の検討
- 細菌性腸管感染症研究の現状 (特集 感染・免疫研究)
- 特集 感染型の食中毒菌
- Disappearance of Glyceraldehyde 3-Phosphate Dehydrogenase from Erythrocyte Membrane by Hemolysis with Thermostable Direct Hemolysin of Vibrio parahaemolyticus or Vibrio cholerae El Tor Hemolysin
- 腸管出血性大腸菌株の室温保存によるVT2遺伝子欠失
- Inhibitory and Killing Activities of Medicinal Plants against Multiple Antibiotic-resistant Helicobacter pylori
- 基礎から臨床へ 大腸菌ベロ毒素の構造と標的細胞に対する作用
- 細菌毒素とは
- Infectious Disease 今話題の炭疽菌について
- 下痢原性細菌
- Demographic Analysis of Breeding Structure in Japanese Thoroughbred Population
- 大腸菌志賀(ベロ)毒素の構造と標的細胞に対する作用 (生物間の攻撃と防御の蛋白質--毒素と生物間相互作用を見直す) -- (第4部 微生物毒素とヒトとの戦い:現状と展開)
- Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157 : H7 derived from the Sakai outbreak
- 新型コレラ
- 腸管感染原因菌,特にビブリオ属菌と病原性大腸菌の病原因子の解析とその応用的研究
- 腸管感染原因菌、特にビブリオ属菌と病原性大腸菌の病原因子の解析とその応用的研究
- Prevention of Bacterial Diarrhea by Pasteurization of Drinking Water in Kenya
- Development of an Enzyme-Labeled Oligonucleotide Probe for Detecting the Escherichia coli Attaching and Effacing A Gene
- Evaluation of Enzyme-Labeled Oligonucleotide Probes to Identify Enterohaemorrhagic Escherichia coli
- Epidemiological Study on Infectious Diarrheal Diseases in Children in a Coastal Rural Area of Kenya
- Effect of Environmental Conditions on Proteins Secreted by Enterohemorrhagic Escherichia coli O26 : H11
- Characterization of Escherichia coli O157 Isolated in Eastern China
- Caseinphosphopeptides (CPP) in Feces of Rats Fed Casein Diet
- Monoclonal Antibody Developed against a Hemolysin of Bacillus thuringiensis
- Effects of Punica granatum Pericarps and Quercus infectoria Nutgalls on Cell Surface Hydrophobicity and Cell Survival of Helicobacter pylori
- Increased Sensitivity of Cholera Toxin Detection by Enzyme-Linked Immunosorbent Assay with Chemical Immobilization of Antibody onto Nylon Surface Providing Hydrophobicity
- Cytotoxicity and Enterotoxicity of the Thermostable Direct Hemolysin-Deletion Mutants of Vibrio parahaemolyticus
- The Gene Encoding the Prepilin Peptidase lnvolved in Biosynthesis of Pilus Colonization Factor Antigen III (CFA/III) of Human Enterotoxigenic Escherichia coli
- An 80μVrms temporal noise 82dB dynamic range CMOS image sensor with a 13 to 19b variable resolution column-parallel folding integration/cyclic ADC (情報センシング・コンシューマエレクトロニクス)
- 故 三輪谷俊夫先生を偲んで
- Demonstration and Characterization of Manganese Superoxide Dismutase of Providencia alcalifaciens
- Gene Dropping Analysis of Ancestral Contributions and Allele Survival in Japanese Thoroughbred Population
- Diagnosis of bacterial enteric infections in children in Zambia
- 新しい食中毒原因菌としてのプロビデンシア・アルカリファシエンス
- 腸炎ビブリオ感染症
- 腸炎ビブリオの分子医学 (特集 消化器感染症と分子医学(1)基礎と臨床--消化管病変を中心に)
- Lateral Transfer of the lux Gene Cluster
- Inhibitory Effects of Active Compounds from Punica granatum Pericarp on Verocytotoxin Production by Enterohemorrhagic Escherichia coli O157:H7
- プロビデンシアの腸管病原性
- ヒト病原細菌を考える
- Nanoimprint Molds with Circumferentially Aligned Patterns Fabricated by Liftoff Process
- Phosphatase assay for multi-phosphorylated substrates using phosphatase specific-motif antibody
- A retrospective analysis comparing the safety and efficacy of chemotherapy in elderly and non-elderly non-small-cell lung cancer patients
- Factors influencing the development of hemolytic uremic syndrome caused by enterohemorrhagic Escherichia coli infection : From a questionnaire survey to in vitro experiment
- Bypass vs. Endovascular Therapy of Infrapopliteal Lesions for Critical Limb Ischemia