チオウラシル類の分析化学的研究(第1報)チオウラシル類の合成と薄層クロマトグラフィー

概要

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The systematic synthesis and thin-layer chromatography (TLC) of S-substituted thiouracil derivatives were carried out to examine the analytical properties of thiouracils. The Rf values on the TLC plate increased with increasing carbon number of alkyl group in S-substituted thiouracil derivatives, using a solyent system of acetone-benzene-MeOH-28%NH_4OH (6 : 2 : 1 : 1). When the copper sulfate-pyridine mixture and of bromine were used as the coloring agent, the spots of purple color appeared very clearly. Subsequently, in order to clarify the relationship between thiouracils and bromine, the reaction chromatography with bromine was carried out. Methylthiouracils on the reaction chromatography plate was separated into some spots using a solvent system of CHCl_3-MeOH-28% NH_4OH (6 : 3 : 1). Among these spots, two spots were identified as 5-bromo-6-methyluracil (VIIIB) and 5,5-dibromo-6-hydroxy-6-methylhydrouracil (IXB). 5,5-Di-bromo-6-methoxy-6-methylhydrouracil (X) was formed by recrystallization of IXB from MeOH.
公益社団法人日本薬学会の論文
1976-09-25