センブリの組織培養の研究(第1報) : カルスの培養条件について

概要

論文の詳細を見る
On modified White's agar medium containing 10% of coconut milk and 1 ppm of 2,4-dichlorophenoxyacetic acid, callus tissues were successfully derived from young root of the sterile seedling of Swertia japonica MAKINO. The callus maintained on the above medium for over two years were used for the determination of the requirements needed for a rapid growth, to give the following results: Without the supplement of coconut milk, by the adequate combination of auxin and kinetin, particularly by the addition of 1 ppm of 2,4-dichlorophenoxyacetic acid, 10 ppm of indole-3-acetic acid or 10 ppm of 1-naphthaleneacetic acid to the medium containing kinetin, a successful growth of callus was obtained. The optimum pH and sucrose concentration were found to be pH 5.5-7.5 and 1-2%, respectively. Illumination inhibited markedly the growth of callus on the medium containing indole-3-acetic acid.
日本生薬学会の論文
1978-03-20