キレート生成能を有する化合物の薄層クロマトグラフィーに関する研究(第8報) : テトラサイクリン類

概要

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Conditions for separatory determination of 11 kinds of tetracycline by thin-layer chromatography were examined. These compounds were found to show tailing on the usual silica gel layer and this was considered to be due to chelation of tetra cyclines with trace metals in the silica gel. In order to stop this reaction, the thin layer was treated with disodium-EDTA and a good result was obtained. Further, changes in the behavior of tetracycline and oxytetracycline by pH and temperature were examined, and the effect of citric acid, boric acid, and calcium ion on these compounds was also examined. At pH 4.0,oxytetracycline undergoes specific decomposition and the solution colors pink. Two kinds of decomposition product were isolated and these were examined by thin-layer chromatorarhy, and by ultraviolet and infrared spectra.
社団法人日本薬学会の論文
1967-05-25