金属蛋白質の研究(第13報) : 銅ヒスチジンキレートのコハク酸酸化酵素に対する影響

概要

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The effects of copper-histidine chelate on succinoxidase activity of liver mitochondria were studied and copper-histidine chelate showed a more marked inhibitory effect than cupric ion alone (Table I). The differences between copper-histidine chelate and cupric ion with regard to inhibitory effect on succinoxidase activity were decreased by treatment of mitochondria at pH 8.5 or by sonic vibration (Table IV). These findings suggested that this inhibitory effect depends on the permeability of mitochondrial membrane to copper-histidine chelate and cupric ion. Therefore, the uptake of copper-histidine chelate and cupric ion by mitochondria was determined by atomic absorption spectroscopy and it was shown that the uptake of cupric ion was slower than that of copper-histidine chelate (Fig.3). In these investigations, it was found that the acceleration of succinoxidase activity was observed in a lower concentration of copper-histidine chelate than that required to inhibit the enzymatic activity. The optimal concentration of copper-histidine chelate to accelerate the enzymatic activity is lower than that of cupric ion. The possible mechanism of rapid uptake of copper-histidine chelate was discussed.
公益社団法人日本薬学会の論文
1967-12-25